Effect of altered matrix proteins on quasilinear viscoelastic properties in transgenic mouse tail tendons
Effect of altered matrix proteins on quasilinear viscoelastic properties in transgenic mouse tail tendons
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DOI：
10.1114/1.1567282
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发表时间：
2003-05-01
影响因子：
3.8
通讯作者：
Soslowsky, LJ
中科院分区：
文献类型：
--
作者：
Elliott, DM;Robinson, PS;Soslowsky, LJ
肌腱具有复杂的力学行为，具有粘弹性、非线性和各向异性。人们普遍认为，这些行为是由组织的组成和结构提供的。然而，很少有数据可以量化这种结构-功能关系。这项研究量化了一组小鼠的肌腱力学行为，包括粘弹性和非线性，这些小鼠的基因工程改变了细胞外基质蛋白。对8周龄Decorin基因敲除组、8周龄还原I型胶原蛋白组、3周龄对照组和8周龄对照组的尾腱束进行单轴拉伸应力松弛实验。用冯氏拟线性粘弹性模型对数据进行了拟合，模型参数代表了线性粘弹性和非线性弹性响应。粘弹性能显示，核心蛋白基因敲除组的应力松弛较大且较快，而三周对照组的应力松弛较小且较慢。8周对照组的弹性参数A明显大于胶原减少组和3周对照组。这项研究为肌腱的结构-功能关系提供了定量证据，包括蛋白多糖在粘弹性中的作用。未来的研究应该直接将成分和结构与肌腱力学联系起来，以设计和评估组织工程化结构或肌腱修复。(C)2003年生物医学工程学会。
Tendons have complex mechanical behaviors that are viscoelastic, nonlinear, and anisotropic. It is widely held that these behaviors are provided for by the tissue's composition and structure. However, little data are available to quantify such structure-function relationships. This study quantified tendon mechanical behaviors, including viscoelasticity and nonlinearity, for groups of mice that were genetically engineered for altered extracellular matrix proteins. Uniaxial tensile stress-relaxation experiments were performed on tail tendon fascicles from the following groups: eight week old decorin knockout, eight week old reduced type I collagen, three week old control, and eight week old control. Data were fit using Fung's quasi-linear viscoelastic model, where the model parameters represent the linear viscoelastic and nonlinear elastic response. The viscoelastic properties demonstrated a larger and faster stress relaxation for the decorin knockout and a smaller and slower stress relaxation for the three week control. The elastic parameter, A, in the eight week control group was significantly greater than in the collagen reduction and three week control groups. This study provides quantitative evidence for structure-function relationships in tendon, including the role of proteoglycan in viscoelasticity. Future studies should directly correlate composition and structure with tendon mechanics for the design and evaluation of tissue-engineered constructs or tendon repairs. (C) 2003 Biomedical Engineering Society.