Roles of Xenopus chemokine ligand CXCLh (XCXCLh) in early embryogenesis.
Roles of Xenopus chemokine ligand CXCLh (XCXCLh) in early embryogenesis.
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非洲爪蟾趋化因子配体 CXCLh (XCXCLh) 在早期胚胎发生中的作用。
DOI：
10.1111/dgd.12432
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Michiue T.
中科院分区：
文献类型：
--
作者：
Goto T.;Ito Y.;Michiue T.
几种趋化因子分子在早期形态发生过程中控制细胞运动。然而，尚不清楚趋化因子分子是否影响细胞命运。在这里，我们在早期胚胎发生过程中鉴定并表征了非洲爪蟾中的CXC型趋化因子配体，非洲爪蟾CXCLh（XCXCLh）。XCXCL在原肠胚阶段在背部植物区表达。背部区域XCXCL的过表达和敲低均抑制原肠胚形成。XCXCLh有助于中内胚层细胞的吸引并加速划痕培养细胞的重新组装。此外，XCXCLh 有助于早期内胚层诱导。 VegTmRNA过表达或高浓度钙离子诱导XCXCLh表达。XCXCLh可能在非洲爪蟾早期胚胎发生过程中的细胞运动和分化中发挥作用。
Several chemokine molecules control cell movements during early morphogenesis. However, it is unclear whether chemokine molecules affect cell fate. Here, we identified and characterized the CXC‐type chemokine ligand inXenopus laevis,Xenopus CXCLh (XCXCLh), during early embryogenesis.XCXCLhis expressed in the dorsal vegetal region at the gastrula stage. Both overexpression and knockdown ofXCXCLhin the dorsal region inhibited gastrulation.XCXCLhcontributed to the attraction of mesendodermal cells and accelerated the reassembly of scratched culture cells. Also,XCXCLhcontributed to early endodermal induction. Overexpression ofVegTmRNA or high concentrations of calcium ions inducedXCXCLhexpression.XCXCLhmay play roles in both cell movements and differentiation during earlyXenopusembryogenesis.