Three-dimensional cell culture device utilizing thin membrane deformation by decompression
Three-dimensional cell culture device utilizing thin membrane deformation by decompression
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DOI：
10.1016/j.snb.2010.01.065
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发表时间：
2010-05-18
影响因子：
8.4
通讯作者：
Suzuki, Osamu
中科院分区：
文献类型：
--
作者：
Anada, Takahisa;Masuda, Taisuke;Suzuki, Osamu
我们开发了一种新的多细胞聚集体(球体)培养装置，它利用聚二甲基硅氧烷薄膜通过减压变形。该装置能够一次快速生产数千个球体，并能调节球体的直径，使球体的尺寸分布窄。球体很容易非侵入性地从装置中取出。该设备的这些特点表明，它具有广泛的应用潜力，如药物筛选和组织工程。(C)2010爱思唯尔B.V.保留所有权利。
We have developed a novel multicellular aggregate (spheroid) culture device that utilizes thin polydimethylsiloxane membrane deformation by decompression. The device was capable of producing thousands of spheroids rapidly at a time and regulating the diameter of spheroids with narrow size distribution. The spheroids were easily retrieved from the device noninvasively. These characteristics of the device suggest that it has the potential for use in a wide variety of applications, such as drug screening and tissue engineering. (C) 2010 Elsevier B.V. All rights reserved.