IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS
IMPROVED SILVER STAINING OF PLANT-PROTEINS, RNA AND DNA IN POLYACRYLAMIDE GELS
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DOI：
10.1002/elps.1150080203
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发表时间：
1987-02-01
期刊：
影响因子：
2.9
通讯作者：
GROSS, HJ
中科院分区：
文献类型：
--
作者：
BLUM, H;BEIER, H;GROSS, HJ
提出了一种新的银染色方法，利用硫代硫酸盐的两种化学性质：通过固定凝胶的预处理来增强图像，以及在图像显影过程中形成可溶性银配合物来防止非特异性背景染色。该方法在无色透明的背景下对纳克范围内的蛋白质、RNA和DNA具有很高的灵敏度。这种方法的性能是通过染色植物叶片蛋白的一维和二维模式记录的。此外，我们首次实现了通过染色程序直接在受感染前质体的一维蛋白模式中检测到非结构性烟草花叶病毒特异性126 kDa蛋白。
A new modification of silver staining is presented which utilizes two chemical properties of thiosulfate: image enhancement by pretreatement of fixed gels, and formation of soluble silver complexes which prevents unspecific background staining during image development. This procedure provides high sensitivity for proteins, RNA and DNA in the nanogram range on a colorless, transparent background. The performance of this method is documented by staining one- and two-dimensional patterns of plant leaf proteins. Moreover, we achieved, for the first time, the detection of the non-structural, tobacco mosaic virus-specific 126 kDa protein directly in the one-dimensional protein pattern of infected proplasts by a staining procedure.