A TRIDECAMER DNA-SEQUENCE SUPPORTS HUMAN MITOCHONDRIAL RNA 3'-END FORMATION INVITRO
A TRIDECAMER DNA-SEQUENCE SUPPORTS HUMAN MITOCHONDRIAL RNA 3'-END FORMATION INVITRO
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DOI：
10.1128/mcb.8.10.4502
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发表时间：
1988-10-01
影响因子：
5.3
通讯作者：
CLAYTON, DA
中科院分区：
文献类型：
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作者：
CHRISTIANSON, TW;CLAYTON, DA
脊椎动物线粒体基因组在 16S rRNA 和亮氨酰-tRNA 基因之间的边界处包含一个假定的转录终止位点。我们之前描述了人类细胞的体外转录系统，具有生成支持3''末端形成的能力，我们证明完全包含在亮氨酰-tRNA基因内的十三聚体序列5''-TGGCAGAGCCCGG-3''对于指导准确终止是必要的。当两个十三聚体序列及其紧邻侧翼区域串联放置时，启动子近端和启动子远端位点均发生终止。此外，含有十三聚体序列的DNA以浓度依赖性方式竞争性抑制终止。这些结果表明，转录终止的序列要求适中，这取决于能够识别十三聚体 DNA 序列存在的因素。
Vertebrate mitochondrial genomes contain a putative transcription termination site at the boundary between the genes for 16S rRNA and leucyl-tRNA. We have described previously an in vitro transcription system form human cells with the capacity to generation to support 3''-end formation, we demonstrated that the tridecamer sequence 5''-TGGCAGAGCCCGG-3'', contained entirely within the gene for leucyl-tRNA, is necessary to direct accurate termination. When two tridecamer sequence and their immediate flanking regions were placed in tandem, termination occured at both promoter-proximal and promoter-distal sites. Furthermore, termination was competitively inhibited, in a concentration-dependent manner, by DNA containing the tridecammer sequence. These results suggest a modest sequence requirement for transcription termination that is contingent on a factor capable of recognizing the presence of the tridecamer DNA sequence.