Dietary choline augments blood choline and cholinergic transmission in terrestrial mollusk Limax maximus.
Dietary choline augments blood choline and cholinergic transmission in terrestrial mollusk Limax maximus.
复制标题
膳食胆碱可增强陆生软体动物最大蜥蜴的血液胆碱和胆碱能传输。
DOI：
10.1152/jn.1982.48.2.451
复制
发表时间：
1982
影响因子：
2.5
通讯作者：
Gelperin,A
中科院分区：
文献类型：
--
作者：
Barry,SR;Gelperin,A
1.用放射酶法测定了陆生软体动物Limax maximus血淋巴中胆碱的浓度，并与对照组进行了比较。蛞蝓血淋巴中游离胆碱的水平随饲料中胆碱的可用性而变化。富含胆碱的动物血浆中的胆碱浓度平均为5.5 μ M，而缺乏胆碱的蛞蝓的平均循环胆碱水平为3.3 μ M。两组之间胆碱水平的差异具有统计学意义。2.在孤立的胆碱能突触之间的唾液爆发神经元（SB）和唾液管肌（SD）的神经生理学测量的传输表明，该突触是敏感的外部胆碱浓度在生理范围内的波动。外源性胆碱从0.5 μ M增加到2.0 μ M，从2.5 μ M增加到5.0 μ M，从5.0 μ M增加到15.0 μ M，随后SB引起的连接电位的幅度增加，从SD记录的局灶性细胞外电极。3.因此，SB-SD突触对外源性胆碱的波动很敏感，这种波动可能会随着饮食的变化而发生。
1. A radioenzymatic assay was used to determine the concentration of choline in the hemolymph of the terrestrial mollusk Limax maximus maintained on choline-enriched and choline-deficient diets. The levels of free choline in the slug's hemolymph varied with the availability of choline in the diet. The concentration of choline in the plasma of choline-enriched animals averaged 5.5 microM while the mean circulating choline level of choline-deprived slugs was 3.3 microM. The difference in choline levels between the two groups was statistically significant. 2. Neurophysiological measurements of transmission at the isolated cholinergic synapses between the salivary burster neuron (SB) and the salivary duct muscle (SD) showed that this synapse was sensitive to fluctuations in the external choline concentration within the physiological range. Increases in exogenous choline from 0.5 to 2.0 microM, from 2.5 to 5.0 microM, and from 5.0 to 15.0 microM were followed by increases in the amplitudes of SB-elicited junction potentials recorded from the SD by a focal extracellular electrode. 3. Thus, the SB-SD synapse is sensitive to the fluctuations in exogenous choline that can occur with a change in diet.