Sex-specific differences in the metabolic enzyme activity and transporter levels in mouse skeletal muscle during postnatal development
Sex-specific differences in the metabolic enzyme activity and transporter levels in mouse skeletal muscle during postnatal development
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出生后发育过程中小鼠骨骼肌代谢酶活性和转运蛋白水平的性别特异性差异
DOI：
10.1139/apnm-2022-0462
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hatta H.
中科院分区：
文献类型：
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作者：
Takahashi K;Kitaoka Y;Hatta H.
尽管成人能量代谢中与性别相关的差异已得到充分表征，但骨骼肌代谢中发育性别特异性的变化在很大程度上是未知的。本研究调查了出生后早期（第 10 天）、断奶后（第 28 天）、性成熟（第 56 天）和成年期（第 140 天）小鼠骨骼肌中高能磷酸盐、糖酵解和线粒体酶活性以及代谢物转运蛋白水平的性别差异。除葡萄糖转运蛋白 (GLUT) 4 水平外，第 10 天和第 28 天没有观察到显着的性别特异性差异。第56天和第140天，雌性小鼠骨骼肌己糖激酶、磷酸果糖激酶和乳酸脱氢酶活性较高，柠檬酸合酶、细胞色素c氧化酶和β-羟酰辅酶A脱氢酶活性低于雄性小鼠。GLUT4和FAT/CD36蛋白水平较高，单羧酸转运蛋白4水平较低。雌性小鼠的骨骼肌高于雄性小鼠，尤其是在第 56 天和第 140 天时。在 140 天龄时，雌性小鼠在跑步机跑步（15 m/min，60 分钟）期间的呼吸交换率低于雄性小鼠，尽管在休息时没有性别差异。总之，性别差异在产后早期和断奶后阶段并不明显，但在小鼠性成熟后变得明显。这些发现表明，性成熟的动物是研究性别差异的更好模型，特别是在研究小鼠能量代谢的背景下。
Although sex-associated differences in energy metabolism in adults are well-characterized, developmental sex-specific changes in skeletal muscle metabolism are largely unknown. This study investigated sex differences in high-energy phosphate, glycolytic, and mitochondrial enzyme activities and metabolite transporter protein levels in mouse skeletal muscles during the early postnatal period (day 10), post-weaning (day 28), sexual maturity (day 56), and adult life (day 140). No significant sex-specific differences were observed on days 10 and 28, except for glucose transporter (GLUT) 4 level. The hexokinase, phosphofructokinase, and lactate dehydrogenase activities of skeletal muscle were higher and the citrate synthase, cytochrome c oxidase, and β-hydroxyacyl-CoA dehydrogenase activities were lower in female mice than those in male mice on days 56 and 140. The GLUT4 and FAT/CD36 protein levels were higher and the monocarboxylate transporter 4 level was lower in the skeletal muscles of female mice than those of male mice, particularly on days 56 and 140. At 140 days of age, the respiratory exchange ratio during treadmill running (15 m/min, 60 min) was lower in females than that in males, despite no sex differences at rest. In summary, sex differences were not evident in the early postnatal and post-weaning periods but became apparent after the mice reached sexual maturity. These findings indicate that sexually mature animals are a better model for investigating sex differences, particularly in the context of studying energy metabolism in mice.