Variable susceptibility of ovarian cancer cells to non-thermal plasma-activated medium.
Variable susceptibility of ovarian cancer cells to non-thermal plasma-activated medium.
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DOI：
10.3892/or.2016.4726
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发表时间：
2016-06
期刊：
影响因子：
4.2
通讯作者：
Kikkawa F
中科院分区：
文献类型：
--
作者：
Utsumi F;Kajiyama H;Nakamura K;Tanaka H;Mizuno M;Toyokuni S;Hori M;Kikkawa F
近年来，低温常压等离子体在包括癌症治疗在内的许多领域得到了广泛的研究。然而，其诱导凋亡的效率有时取决于细胞种类和实验条件而变化。本研究的目的是阐明是什么原因导致这些差异的反应等离子体治疗。4种卵巢癌细胞株的增殖抑制率（PIR）均受细胞密度的影响，其中OVCAR-3和NOS 2细胞的PIR受细胞密度的影响更为明显。此外，TOV 21 G和ES-2细胞对等离子体活化培养基（PAM）的敏感性显著高于其他两种细胞系。我们证明，活性氧的比例和细胞数量对PAM对卵巢癌细胞的作用有显着影响。此外，细胞形态特征也与细胞的生长发育密切相关
Non-thermal atmospheric pressure plasma has been widely studied in recent years in many fields, including cancer treatment. However, its efficiency for inducing apoptosis sometimes varies depending on the cell species and experimental conditions. The aim of this study was to elucidate what causes these differences in responses to plasma treatment. Using four ovarian cancer cell lines, the cell density had a markedly negative impact on the proliferation inhibition rate (PIR) and it was more obvious in OVCAR-3 and NOS2 cells. Furthermore, TOV21G and ES-2 cells were drastically sensitive to plasma-activated medium (PAM) compared with the other two cell lines. We demonstrated that the proportion of reactive oxygen species and cell number had a marked impact on the effect of PAM against ovarian cancer cells. Additionally it was suggested that the morphological features of cells were also closely related