Expression of GABAA receptor subunits in rat brainstem auditory pathways:: Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus
Expression of GABAA receptor subunits in rat brainstem auditory pathways:: Cochlear nuclei, superior olivary complex and nucleus of the lateral lemniscus
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DOI：
10.1016/s0306-4522(00)00525-x
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Merlo, D
中科院分区：
文献类型：
--
作者：
Campos, ML;De Cabo, C;Merlo, D
GABA的抑制对于听觉处理是重要的，但离子型A受体的任何适应都是未知的。在这里，我们描述，使用原位杂交，GABAA受体在大鼠耳蜗核，上级橄榄复合体，背侧和腹侧的外侧丘系核的亚单位的表达模式。所有神经元表达β 3和γ 2L亚基信使RNA，但使用不同的α亚基。在耳蜗背核，梭形（锥体）和巨细胞表达α 1，α 3，β 3和γ 2L。背侧耳蜗核中间神经元，特别是垂直或turboculoventral细胞和cartwheel细胞，表达α 3，β 3和γ 2L。在腹侧耳蜗核，章鱼细胞表达α 1，β 3，γ 2L和δ。球形细胞表达α 1、α 3、α 5、β 3和γ 2L。在上级橄榄复合体中，表达谱为α 3、α 5、β 3和γ 2L。背侧和腹侧耳蜗核颗粒细胞表达α 1，α 6，β 3和γ 2L;与小脑颗粒细胞不同，它们不表达β 2，γ 2S或δ亚基基因。耳蜗核颗粒细胞中δ亚基的缺失可能意味着突触外GABAA受体介导的被膜抑制对这种细胞类型不太重要。在外侧丘系的背侧核和腹侧核中，α 1、β 3和γ 2L是主要的亚基信使RNA，腹侧核也表达δ亚基。与许多脑区相反，β 2亚基基因和γ 2S剪接形式在听觉脑干核中不高表达。含有β 3和γ 2L的GABA（A）受体可能特别适合于听觉处理，这可能是因为这种亚基组合的独特磷酸化特征。(C)2001年IBRO。由Elsevier Science Ltd.出版，版权所有。
Inhibition by GABA is important For auditory processing, but any adaptations of the ionotropic type A receptors are unknown. Here we describe, using in situ hybridization, the subunit expression patterns of GABAA receptors in the rat cochlear nucleus, superior olivary complex, and dorsal and ventral nuclei of the lateral lemniscus. All neurons express the beta3 and gamma 2L subunit messenger RNAs, but use different ct subunits. In the dorsal cochlear nucleus, fusiform (pyramidal) and giant cells express alpha1, alpha3, beta3 and gamma 2L. Dorsal cochlear nucleus interneurons, particularly vertical or turboculoventral cells and cartwheel cells, express alpha3, beta3 and gamma 2L. In the ventral cochlear nucleus, octopus cells express alpha1, beta3, gamma 2L and delta. Spherical cells express alpha1, alpha3, alpha5, beta3 and gamma 2L. In the superior olivary complex, the expression profile is alpha3, alpha5, beta3 and gamma 2L. Both dorsal and ventral cochlear nucleus granule cells express alpha1, alpha6, beta3 and y2L; unlike their cerebellar granule cell counterparts, they do not express beta2, gamma 2S or the delta subunit genes. The delta subunit's absence from cochlear nucleus granule cells may mean that tunic inhibition mediated by extrasynaptic GABAA receptors is less important for this cell type. In both the dorsal and ventral nuclei of the lateral lemniscus, alpha1, beta3 and y2L are the main subunit messenger RNAs; the ventral nucleus also expresses the delta subunit.We have mapped, using in situ hybridization, the subunit expression patterns of the GABA(A) receptor in the auditory brainstem nuclei. In contrast to many brain regions, the beta2 subunit gene and gamma 2S splice forms are not highly expressed in auditory brainstem nuclei. GABA(A) receptors containing beta3 and y2L may be particularly well suited to auditory processing, possibly because of the unique phosphorylation profile of this subunit combination. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.