Osteopontin mediates necroptosis in lung injury after transplantation of ischaemic renal allografts in rats
Osteopontin mediates necroptosis in lung injury after transplantation of ischaemic renal allografts in rats
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骨桥蛋白介导大鼠缺血同种异体肾移植后肺损伤中的坏死性凋亡
DOI：
10.1016/j.bja.2019.05.041
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发表时间：
2019
影响因子：
9.8
通讯作者：
Ma Daqing
中科院分区：
文献类型：
--
作者：
Zhao Hailin;Chen Qian;Huang Han;Suen Ka C.;Alam Azeem;Cui Jiang;Ciechanowicz Sarah;Ning Jiaolin;Lu Kaizhi;Takata Masao;Gu Jianteng;Ma Daqing
利用药用植物进行生态友好的绿色纳米颗粒合成，由于其潜在的治疗用途而获得了极大的重视。本研究以白云木叶和根的水提物为原料，在室温下合成了纳米银。采用MTT法研究AgNPs对宫颈癌细胞系（HeLa）、乳腺癌细胞系（MCF-7）和小鼠胚胎成纤维细胞（NIH-3 T3）的抗癌作用，评价其毒性。用稳定的DPPH自由基评价其抗氧化能力。荧光显微镜下通过DAPI和PI染色，观察AgNPs在更敏感的HeLa细胞中引起的核凋亡变化。采用多种技术对生物合成AgNPs的理化性质进行了表征。AgNPs在很短的时间内形成，紫外-可见光谱显示AgNPs的特征吸收峰。SEM和TEM显示AgNPs呈球形，XRD显示其结晶性质。EDX分析显示，绿色合成AgNPs中银的比例很高。FTIR分析表明次级代谢物参与了AgNPs的合成。体外毒性和抗氧化研究表明，中药和生物合成AgNPs具有明显的剂量依赖性和时间依赖性的抗癌和抗氧化潜能。此外，正常细胞系的研究和细胞凋亡的显微分析表明，与顺铂相比，AgNPs具有良好的安全性，并诱导显著的细胞凋亡作用。根据目前的研究结果，人们强烈认为使用j。dolomiaea提供大规模生产的生物相容性AgNPs，可作为抗癌药物替代癌细胞系。
Eco-friendly green synthesis of nanoparticles using medicinal plants gained immense importance due to its potential therapeutic uses. In the current study, silver nanoparticles (AgNPs) were synthesized using water extract ofJurinea dolomiaealeaf and root at room temperature. MTT assay was used to study anticancer potential of AgNPs against cervical cancer cell line (HeLa), breast cancer cell lines (MCF-7), and mouse embryonic fibroblast (NIH-3 T3) cell line for toxicity evaluation. The antioxidant potential was evaluated using stable DPPH radicals. In addition, the apoptotic nuclear changes prompted by AgNPs in more susceptible HeLa cells were observed using fluorescence microscope through DAPI and PI staining. Physiochemical properties of biosynthesized AgNPs were characterized using various techniques. AgNPs were formed in very short time and UV–vis spectra showed characteristic absorption peak of AgNPs. SEM and TEM showed spherical shape of AgNPs and XRD revealed their crystalline nature. EDX analysis revealed high percentage of silver in green synthesized AgNPs. FTIR analysis indicated involvement of secondary metabolites in fabrication of AgNPs.In vitrocytotoxic and antioxidant study revealed that herb and biosynthesized AgNPs exhibited significant dose-dependent and time-dependent anticancer and antioxidant potential. Furthermore, study on normal cell line and microscopic analysis of apoptosis revealed that AgNPs exhibited good safety profile as compared to cisplatin and induces significant apoptosis effect. Based on the current findings, it is strongly believe that use ofJ. dolomiaeaoffers large scale production of biocompatible AgNPs that can be used as alternative anticancer agents against cancer cell lines tested.