The transcription factor MML4_D12 regulates fiber development through interplay with the WD40-repeat protein WDR in cotton
The transcription factor MML4_D12 regulates fiber development through interplay with the WD40-repeat protein WDR in cotton
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棉花中转录因子 MML4_D12 通过与 WD40 重复蛋白 WDR 相互作用调节纤维发育
DOI：
10.1093/jxb/eraa104
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发表时间：
2020-06-22
影响因子：
6.9
通讯作者：
Zhang, Tianzhen
中科院分区：
文献类型：
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作者：
Tian, Yue;Du, Jingjing;Zhang, Tianzhen
在植物中，一种重要的调节复合物 MYB-碱性螺旋-环-螺旋 (bHLH)-WD40 (MBW) 参与拟南芥中毛状体的发育和花青素和原花青素的合成。通常，WD40 蛋白为 MYB 和 bHLH 蛋白之间的蛋白间相互作用提供支架。 R2R3 MYB 转录因子第 9 亚组的成员（包括对植物细胞分化很重要的 MYBMIXTA 样 (MML) 基因）无法与 bHLH 相互作用。在这项研究中，我们报道了棉花（Gossypium hirsutum）种子毛状体或皮棉纤维相关的 GhMML 因子 GhMML4_D12 与分歧的 WD40 蛋白（GhWDR）相互作用，其过程类似于拟南芥毛状体发育中涉及的 MBW 三元复合物，但又不同。 GhMML4_D12 的氨基酸 250-267 和 GhWDR 的第一和第三 WD40 重复结构域决定了它们的相互作用。 GhWDR 可以将拟南芥 ttg1 恢复为野生型，证实其在毛状体发育中的直系同源功能。我们的研究结果有助于了解 MML 和 WD40 家族在植物和提高棉纤维生产中的关键作用。
In planta, a vital regulatory complex, MYB-basic helix-loop-helix (bHLH)-WD40 (MBW), is involved in trichome development and synthesis of anthocyanin and proanthocyanin in Arabidopsis. Usually, WD40 proteins provide a scaffold for protein-protein interaction between MYB and bHLH proteins. Members of subgroup 9 of the R2R3 MYB transcription factors, which includes MYBMIXTA-Like (MML) genes important for plant cell differentiation, are unable to interact with bHLH. In this study, we report that a cotton (Gossypium hirsutum) seed trichome or lint fiber-related GhMML factor, GhMML4_D12, interacts with a diverged WD40 protein (GhWDR) in a process similar to but different from that of the MBW ternary complex involved in Arabidopsis trichome development. Amino acids 250-267 of GhMML4_D12 and the first and third WD40 repeat domains of GhWDR determine their interaction. GhWDR could rescue Arabidopsis ttg1 to its wild type, confirming its orthologous function in trichome development. Our findings shed more light towards understanding the key role of the MML and WD40 families in plants and in the improvement of cotton fiber production.