Localization of substance P, CGRP, VIP, neuropeptide Y, and somatostatin immunoreactive nerve fibers in the carotid labyrinths of some amphibian species
Localization of substance P, CGRP, VIP, neuropeptide Y, and somatostatin immunoreactive nerve fibers in the carotid labyrinths of some amphibian species
复制标题
一些两栖动物颈动脉迷路中 P 物质、CGRP、VIP、神经肽 Y 和生长抑素免疫反应性神经纤维的定位
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
S. Tsuji
中科院分区：
文献类型：
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作者：
T. Kusakabe;P. Anglade;S. Tsuji
摘要采用过氧化物酶-抗过氧化物酶法，比较了几种两栖动物颈动脉迷宫中P物质（SP）、CGRP、VIP、神经肽Y （NPY）和生长抑素（SOM）的免疫组化定位。在颈动脉迷路血管间质神经纤维中发现SP、CGRP、VIP、NPY的免疫反应性。SP、CGRP和VIP免疫反应性静脉曲张纤维密集分布于颈动脉迷路周围部分。部分sp免疫反应纤维与cgrp免疫反应纤维分布相似。NPY和SOM免疫反应性静脉曲张纤维密度低。迷宫内未见脑啡肽免疫反应性。这些肽的强度因物种而异。血管球细胞对所研究的7种肽均未表现出免疫反应性。这些结果表明，颈动脉迷宫管的血管调节功能除了通过血管球和平滑肌细胞的亲密接触（g-s连接）进行调节外，还受肽能机制的控制，这可能是颈动脉迷宫管的功能之一。
SummaryImmunohistochemical localization of substance P (SP), CGRP, VIP, neuropeptide Y (NPY), and somatostatin (SOM) in the carotid labyrinth were compared in some species of amphibians using the peroxidase-antiperoxidase method. Immunoreactivity of SP, CGRP, VIP, and NPY was found in the nerve fibers distributed in the intervascular stroma of the carotid labyrinth. SP, CGRP, and VIP immunoreactive varicose fibers were densely distributed in the peripheral portion of the carotid labyrinth. Some SP-immunoreactive fibers were distributed similarly to CGRP-immunoreactive fibers. The density of NPY and SOM immunoreactive varicose fibers was low. No immunoreactivity of enkephalins was observed in the labyrinth. The intensities of these peptides were varied from species to species. No glomus cells showed immunoreactivity for any of the 7 peptides studied. These results suggest that the vascular regulatory function, which is one of the possible functions of the carotid labyrinth, is controlled by the peptidergic mechanisms in addition to regulation through intimate apposition of glomus and smooth muscle cells (g-s connection).