The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility.
The Cannabinoid Receptor Interacting Proteins 1 of zebrafish are not required for morphological development, viability or fertility.
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DOI：
10.1038/s41598-017-05017-5
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发表时间：
2017-07-07
影响因子：
4.6
通讯作者：
Hughes SM
中科院分区：
文献类型：
--
作者：
Fin L;Bergamin G;Steiner RA;Hughes SM
大麻素受体相互作用蛋白1 （Cnrip1）被发现与大麻素受体1 （CB1R，也称为Cnr1或CB1）的细胞内区域相互作用。小鼠的功能分析显示大麻素敏感性变化，Cnrip1最近被认为可以控制非洲爪蟾的眼睛发育。在斑马鱼中描述了两个Cnrip1基因，cnrip1a和cnrip1b。原位mRNA杂交显示，编码每个基因的mRNA主要在脑和脊髓中积累，但也在其他地方积累。例如，在骨骼肌形成过程中表达cnrip1b。CRISPR/Cas9基因组编辑在cnrip1a和cnrip1b中产生了预测的零突变。每个突变都触发了相应mRNA转录物的无义介导的衰变。在两个突变体中均未观察到形态或行为表型。此外，在母系和合子中同时缺乏Cnrip1a和Cnrip1b的鱼类是可存活和可生育的，尽管在至少400 Myr的进化保守性很强，但迄今为止还没有发现表型。
The Cannabinoid Receptor Interacting Protein 1 (Cnrip1) was discovered as an interactor with the intracellular region of Cannabinoid Receptor 1 (CB1R, also known as Cnr1 or CB1). Functional assays in mouse show cannabinoid sensitivity changes and Cnrip1 has recently been suggested to control eye development in Xenopus laevis. Two Cnrip1 genes are described in zebrafish, cnrip1a and cnrip1b. In situ mRNA hybridisation revealed accumulation of mRNA encoding each gene primarily in brain and spinal cord, but also elsewhere. For example, cnrip1b is expressed in forming skeletal muscle. CRISPR/Cas9 genome editing generated predicted null mutations in cnrip1a and cnrip1b. Each mutation triggered nonsense-mediated decay of the respective mRNA transcript. No morphological or behavioural phenotype was observed in either mutant. Moreover, fish lacking both Cnrip1a and Cnrip1b both maternally and zygotically are viable and fertile and no phenotype has so far been detected despite strong evolutionary conservation over at least 400 Myr.
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影响因子：
4.7
作者：
Blume LC;Leone-Kabler S;Luessen DJ;Marrs GS;Lyons E;Bass CE;Chen R;Selley DE;Howlett AC
通讯作者：
Howlett AC
影响因子：
13.8
作者：
Maccarrone, Mauro;Bab, Rai;Biro, Tamas;Cabral, Guy A.;Dey, Sudhansu K.;Di Marzo, Vincenzo;Konje, Justin C.;Kunos, George;Mechoulam, Raphael;Pacher, Pal;Sharkey, Keith A.;Zimmer, Andreas
通讯作者：
Zimmer, Andreas
影响因子：
3.1
作者：
Guggenhuber, Stephan;Alpar, Alan;Klugmann, Matthias
通讯作者：
Klugmann, Matthias
影响因子：
4.8
作者：
Anavi-Goffer, Sharon;Fleischer, Daniel;Abood, Mary E.
通讯作者：
Abood, Mary E.
影响因子：
3.6
作者：
Blume, Lawrence C.;Patten, Theresa;Howlett, Allyn C.
通讯作者：
Howlett, Allyn C.