IDENTIFICATION OF A PROPOSED NEW SEROVAR OF ACTINOBACILLUS PLEUROPNEUMONIAE: SEROVAR 16
IDENTIFICATION OF A PROPOSED NEW SEROVAR OF ACTINOBACILLUS PLEUROPNEUMONIAE: SEROVAR 16
复制标题
DOI：
10.1556/004.2015.041
复制
发表时间：
2015-12-01
影响因子：
0.9
通讯作者：
Fodor, Laszlo
中科院分区：
文献类型：
--
作者：
Sarkoezi, Rita;Makrai, Laszlo;Fodor, Laszlo
从匈牙利猪胸膜肺炎的病理病变中分离到的5株胸膜肺炎放线杆菌不能被归类到接受的15个血清型中的任何一个。用这些不能分型的胸膜肺炎沙门氏菌免疫兔血清，间接血凝试验证明它们形成了一个独特的群，它们与胸膜肺炎沙门氏菌的模式株无交叉反应。5株菌株均含有毒素相关基因apxIA和apxIB，分别与ApxI、ApxII表达和最大长度(2800bp)的apxIV基因有关。碳源利用模式和16S rRNA基因序列分析证实了建议的模式株胸膜肺炎弧菌A-85/14的种类鉴定。
Five Actinobacillus pleuropneumoniae strains isolated from pathological lesions of porcine pleuropneumonia in Hungary could not be assigned to any of the accepted 15 serovars. Using hyperimmune serum raised against these untypable-serovar A. pleuropneumoniae strains in rabbits, indirect haemagglutination tests proved that they form a distinct group and there is no cross-reaction between them and the type strains of A. pleuropneumoniae. All five strains harboured the toxin-associated genes for the production (apxIA) and secretion (apxIB) of ApxI, the gene for the expression of ApxII and the largest-size (2800 bp) apxIV gene. The carbon source utilisation pattern and the sequence analysis of the 16S rRNA gene confirmed the species identification of the suggested type strain, A. pleuropneumoniae A-85/14. A new serovar of A. pleuropneumoniae - serovar 16 - is proposed with A. pleuropneumoniae A-85/14 as reference strain.