Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain
Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain
复制标题
DOI：
10.1016/j.bbrc.2004.06.139
复制
发表时间：
2004-08-20
影响因子：
3.1
通讯作者：
Okabe, M
中科院分区：
文献类型：
--
作者：
Matsumura, H;Hasuwa, H;Okabe, M
我们已经建立了一个转基因小鼠品系，在其中的foxed新霉素抗性盒插入之间的CA G启动子和EGFP。当这些转基因小鼠与表达Cre的转基因动物交配时，获得的后代呈荧光绿色。然后，我们建立了一个转基因小鼠系，其中上述构建体中的EGFP被白喉毒素A链（DT）取代。当后者的转基因小鼠与表达Cre限制生殖细胞的小鼠交配时，我们获得了健康但不育的后代，这是由于DT表达破坏了生殖细胞系。我们预测，这一策略将是有用的，为人类疾病，具有各种缺失的细胞谱系与DT中断产生的新的动物模型的建设。(C)2004年爱思唯尔公司All rights reserved.
We have established a transgenic mouse line in which foxed neomycin resistant cassette was inserted between the CA G promoter and EGFP. When these transgenic mice were mated with Cre-expressing transgenic animals, the offspring obtained were fluorescent green. We then established a transgenic mouse line in which EGFP in the above construct was replaced by diphtheria toxin A chain (DT). When the latter transgenic mice were mated with mice expressing Cre restricted to germ cells, we obtained healthy but sterile offspring due to a disruption of germ line cells by DT expression. We predict that this strategy will be useful for the construction of new animal models for human diseases, featuring a variety of missing cell lineages produced by disruption with DT. (C) 2004 Elsevier Inc. All rights reserved.