Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance
Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance
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DOI：
10.1096/fj.08-108266
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发表时间：
2008-11-01
期刊：
影响因子：
4.8
通讯作者：
Kozak, Leslie P.
中科院分区：
文献类型：
--
作者：
Nikonova, Larissa;Koza, Robert A.;Kozak, Leslie P.
C57BL/6J （B6）小鼠饲喂致肥性饮食后，白色脂肪中中胚层特异性转录基因（Mest） mRNA和蛋白水平发生了50倍的变异，与脂肪量的增加呈正相关。MEST蛋白仅在脂肪细胞中检测到，其诱导发生在不饱和和饱和膳食脂肪中。为了验证MEST调节脂肪膨胀的假设，将其表达与暴露于饮食和环境温度的B6小鼠中硬脂酰辅酶a去饱和酶（Scd1）的表达进行了比较，这些饮食和环境温度产生了分离食物摄入和肥胖影响的条件。在一系列条件下，Mest的表达总是与肥胖的变化有关，而Scd1的表达与饮食中饱和脂肪的含量有关。Mest mRNA在出生后生长早期脂肪团扩张最迅速阶段的开始时表达水平最高。MEST定位于内质网/高尔基体，根据其与α / β折叠水解酶超家族成员的序列同源性预测，其作为脂肪酶或酰基转移酶的酶学特性可以使其在正能量平衡条件下参与脂质积累。在基因相同的小鼠中，肥胖和Mest表达的变化也提供了表观遗传调控的模型。-尼科诺娃，L.，科扎，R. A.，门多萨，T.，赵，P.-M。中胚层特异性转录与脂肪质量扩张有关，以响应正能量平衡。中华医学杂志22,3925-3937 （2008）
A 50-fold variation in mRNA and protein levels of the mesoderm-specific transcript gene (Mest) in white fat of C57BL/6J (B6) mice fed an obesogenic diet is positively correlated with expansion of fat mass. MEST protein was detected only in adipocytes, in which its induction occurred with both unsaturated and saturated dietary fat. To test the hypothesis that MEST modulates fat mass expansion, its expression was compared to that of stearoyl CoA desaturase (Scd1) in B6 mice exposed to diets and environmental temperatures that generated conditions separating the effects of food intake and adiposity. Under a range of conditions, Mest expression was always associated with variations in adiposity, whereas Scd1 expression was associated with the amount of saturated fat in the diet. Mest mRNA was expressed at its highest levels during early postnatal growth at the onset of the most rapid phase of fat mass expansion. MEST is localized to the endoplasmic reticulum/Golgi apparatus where its putative enzymatic properties as a lipase or acyltransferase, predicted from sequence homology with members of the alpha/beta fold hydrolase superfamily, can enable it to function in lipid accumulation under conditions of positive energy balance. Variations in adiposity and Mest expression in genetically identical mice also provides a model of epigenetic regulation. -Nikonova, L., Koza, R. A., Mendoza, T., Chao, P.-M., Curley, J. P., Kozak, L. P. Mesoderm-specific transcript is associated with fat mass expansion in response to a positive energy balance. FASEB J. 22, 3925-3937 (2008)