The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.
The intracellular immune receptor Rx1 regulates the DNA-binding activity of a Golden2-like transcription factor.
复制标题
DOI：
10.1074/jbc.ra117.000485
复制
发表时间：
2018-03-02
期刊：
影响因子：
--
通讯作者：
Cann MJ
中科院分区：
文献类型：
--
作者：
Townsend PD;Dixon CH;Slootweg EJ;Sukarta OCA;Yang AWH;Hughes TR;Sharples GJ;Pålsson LO;Takken FLW;Goverse A;Cann MJ
植物富含亮氨酸重复序列（NLR）的核苷酸结合蛋白使免疫系统能够识别和响应病原体的攻击。免疫激活的早期结果是转录重编程，并且一些NLR已被证明在细胞核中起作用并与转录因子相互作用。马铃薯的Rx1 NLR蛋白还能够结合并扭曲双链DNA。然而，支持Rx1在DNA转录重编程中的作用的Rx1宿主靶标是未知的。在这里，我们报告的Rx1和NbGlk1，一个黄金2样转录因子之间的功能相互作用。Rx1在体外和植物中与NbGlk1结合。NbGlk1与已知的Golden2样共有DNA序列结合。Rx1在体外降低NbGlk1对DNA的结合亲和力。NbGlk1激活对马铃薯X病毒的细胞反应，而Rx1与NbGlk1结合并阻止其在植物DNA上的组装，除非被PVX激活。这项研究为NLR如何在病原体感知后协调DNA上的免疫信号传导反应提供了新的机制见解。
Plant nucleotide-binding leucine–rich repeat (NLR) proteins enable the immune system to recognize and respond to pathogen attack. An early consequence of immune activation is transcriptional reprogramming, and some NLRs have been shown to act in the nucleus and interact with transcription factors. The Rx1 NLR protein of potato is further able to bind and distort double-stranded DNA. However, Rx1 host targets that support a role for Rx1 in transcriptional reprogramming at DNA are unknown. Here, we report a functional interaction between Rx1 and NbGlk1, a Golden2-like transcription factor. Rx1 binds to NbGlk1 in vitro and in planta. NbGlk1 binds to known Golden2-like consensus DNA sequences. Rx1 reduces the binding affinity of NbGlk1 for DNA in vitro. NbGlk1 activates cellular responses to potato virus X, whereas Rx1 associates with NbGlk1 and prevents its assembly on DNA in planta unless activated by PVX. This study provides new mechanistic insight into how an NLR can coordinate an immune signaling response at DNA following pathogen perceptions.