Extracellular mechanical forces drive endocardial cell volume decrease during zebrafish cardiac valve morphogenesis
Extracellular mechanical forces drive endocardial cell volume decrease during zebrafish cardiac valve morphogenesis
复制标题
斑马鱼心脏瓣膜形态发生过程中细胞外机械力驱动心内膜细胞体积减少
DOI：
10.1016/j.devcel.2022.02.011
复制
发表时间：
2022
影响因子：
11.8
通讯作者：
Vermot Julien
中科院分区：
文献类型：
--
作者：
Vignes Helene;Vagena-Pantoula Christina;Prakash Mangal;Fukui Hajime;Norden Caren;Mochizuki Naoki;Jug Florian;Vermot Julien
器官形态发生涉及组织特性的动态变化，而细胞通过机械敏感途径适应其机械环境。在形态发生过程中，机械信号如何影响细胞行为仍不清楚。在这里，我们研究了心脏瓣膜发育的斑马鱼房室管（AVC）的形成。我们发现，AVC的形式与增加激活的肌动球蛋白网络和细胞取向的变化与组织收敛区。我们证明，组织收敛发生与机械力和机械敏感通道TRPP 2/TRPV 4触发的细胞体积的减少。最后，我们表明，细胞外基质成分透明质酸控制细胞体积的变化。总之，我们的数据表明，多个力敏感信号通路汇聚在一起，以调节细胞体积。我们的结论是，细胞体积减小是心血管形态发生过程中机械转导激活的一个关键细胞特征。这项工作进一步确定了机械力和细胞外基质如何影响发育中器官的组织重塑。
Organ morphogenesis involves dynamic changes of tissue properties while cells adapt to their mechanical environment through mechanosensitive pathways. How mechanical cues influence cell behaviors during morphogenesis remains unclear. Here, we studied the formation of the zebrafish atrioventricular canal (AVC) where cardiac valves develop. We show that the AVC forms within a zone of tissue convergence associated with the increased activation of the actomyosin meshwork and cell-orientation changes. We demonstrate that tissue convergence occurs with a reduction of cell volume triggered by mechanical forces and the mechanosensitive channel TRPP2/TRPV4. Finally, we show that the extracellular matrix component hyaluronic acid controls cell volume changes. Together, our data suggest that multiple force-sensitive signaling pathways converge to modulate cell volume. We conclude that cell volume reduction is a key cellular feature activated by mechanotransduction during cardiovascular morphogenesis. This work further identifies how mechanical forces and extracellular matrix influence tissue remodeling in developing organs.