Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung.
Isolation and Purification of Epithelial and Endothelial Cells from Mouse Lung.
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DOI：
10.1007/978-1-4939-7896-0_6
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Cook DN
中科院分区：
文献类型：
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作者：
Nakano H;Nakano K;Cook DN
对吸入的环境因子的反应由多种免疫细胞类型的协调作用控制，包括巨噬细胞、树突细胞和淋巴细胞。最近的证据表明，一些结构细胞也可以促进免疫反应的启动和传播。例如，气道上皮细胞可促进嗜酸性粒细胞炎症以响应变应原吸入。然而，关于这些细胞类型中的每一种如何与其他细胞相互作用，以及这些相互作用如何塑造对吸入剂的免疫反应，还有很多东西有待了解。由于缺乏可靠的方法从同一组织样本中分离出多个不同的细胞群，这类研究受到了阻碍。因此，研究人员不得不在使用不同动物的不同方案来分离不同的细胞群体之间进行选择，或者接受对于某些群体，细胞产量非常低的事实。为了克服这些困难，我们开发了一种方便实用的方法，从小鼠肺中分离和纯化上皮细胞和内皮细胞亚群。在这里，我们详细描述这些方法。
Responses to inhaled environmental agents are controlled by the coordinated actions by multiple immune cell types, including macrophages, dendritic cells and lymphocytes. Recent evidence indicates that some structural cells can also contribute to the initiation and propagation of immune responses. For example, airway epithelial cells can promote eosinophilic inflammation in response to upon allergen inhalation. Much remains to be learned, however, regarding how each of these cell types interacts with the others, and how these interactions shape immune responses to inhaled agents. Such studies have been hampered by the lack of reliable methods to isolate multiple and distinct populations of cells from the same tissue sample. Consequently, investigators have had to choose between using different protocols with different animals to isolate different populations of cells, or accept that for some populations, cell yields are very low. To overcome these difficulties, we have developed a convenient and practical method to isolate and purify subpopulations of epithelial and endothelial cells from mouse lung. Here, we describe these methods in detail.