An alternative cytoplasmic domain of the integrin beta 3 subunit.
An alternative cytoplasmic domain of the integrin beta 3 subunit.
复制标题
DOI：
10.1073/pnas.86.14.5415
复制
发表时间：
1989-07
影响因子：
11.1
通讯作者：
T. H. Kuppevelt;L. Languino;J. Gailit;Susumu Suzuki;E. Ruoslahti
中科院分区：
文献类型：
--
作者：
T. H. Kuppevelt;L. Languino;J. Gailit;Susumu Suzuki;E. Ruoslahti
通过用β 3（gpIIIa）DNA探针筛选，从胎盘cDNA文库中分离出编码血小板整合素gpIIb/IIIa和玻连蛋白受体的共享β亚基（β 3）的新形式的cDNA。这种β 3变体与先前报道的β 3不同之处在于胞质结构域短8个氨基酸，并且具有替代的13个氨基酸的COOH末端肽。该cDNA的3'端非翻译区也不同于以前报道的序列，而编码跨膜区和胞外区的区域与之相同。用逆转录结合聚合酶链反应（RT-PCR）的方法证明人胎盘组织和两个人细胞系中含有该变异mRNA。先前已知的β 3和本文描述的变体β 3的cDNA序列表明，这些亚基的胞质结构域之间的差异是由于mRNA剪接的结果。这些胞质结构域可以为β 3整联蛋白与细胞骨架组分相互作用提供替代手段。
A cDNA encoding a new form of the shared beta subunit (beta 3) of the platelet integrin gpIIb/IIIa and the vitronectin receptor was isolated from a placental cDNA library by screening with a beta 3 (gpIIIa) DNA probe. This beta 3 variant differs from the previously reported beta 3 in that the cytoplasmic domain is 8 amino acids shorter and has an alternative, 13-amino acid COOH-terminal peptide. The 3' untranslated region of the cDNA also differs from the previously reported sequence, while the region coding for the transmembrane domain and extracellular domain is identical to it. Reverse transcription combined with polymerase chain reaction was used to show that human placental tissue and two human cell lines contain the variant mRNA. The sequences of the cDNAs for the previously known beta 3 and the variant beta 3 described here suggest that the difference between the cytoplasmic domains of these subunits arises as a result of an alternative mRNA splicing. These cytoplasmic domains may provide alternative means for the beta 3 integrins to interact with cytoskeletal components.