Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production
Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production
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DOI：
10.1016/j.biortech.2010.10.048
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发表时间：
2011-02-01
影响因子：
11.4
通讯作者：
Subramanian, Rangaswamy
中科院分区：
文献类型：
--
作者：
Nakkeeran, Ekambaram;Umesh-Kumar, Sukumaran;Subramanian, Rangaswamy
当通过深层发酵和固态发酵生长时，碳曲霉产生不同分子形式的多聚半乳糖醛酸酶（PG：EC 3.2.1.15），其中42 kDa PG具有7000 U/mg蛋白质的高比活性。采用膜分离和海藻酸盐亲和沉淀两种方法对酶进行纯化，两种方法对酶的浓缩形式不同。AAP工艺选择性地纯化和浓缩高活性PG，而IMP产生不同的PG以及淀粉酶和蛋白酶。在通常商业上使用的条件下，对用于苹果汁制备的AAP酶制剂的评价表明，高活性PG不会导致良好的果汁澄清度。用IMP加工的酶，果汁产量和澄清度与用来自A.尼日尔. (C)2010爱思唯尔有限公司版权所有。
Aspergillus carbonarius, when grown by submerged and solid-state fermentation, produces different molecular forms of polygalacturonase (PG: EC 3.2.1.15), among them a 42 kDa PG with a high specific activity of 7000 U/mg protein. When the enzymes were purified by integrated membrane process (IMP) and alginate affinity precipitation (AAP), the two processes concentrated different forms of the enzyme. The AAP process selectively purified and concentrated the high active PG whereas the IMP yielded different PGs and also amylase and protease. Evaluation of the AAP enzyme preparations for apple juice preparation under conditions usually employed commercially demonstrated that the high activity PG did not result in good juice clarity. With IMP processed enzymes, juice yields and clarity were similar to that obtained with commercial PG from A. niger. (C) 2010 Elsevier Ltd. All rights reserved.