Identification of genes responding to nematode infection in red grouse.
Identification of genes responding to nematode infection in red grouse.
复制标题
红松鸡线虫感染响应基因的鉴定。
DOI：
10.1111/j.1755-0998.2010.02912.x
复制
发表时间：
2011
影响因子：
7.7
通讯作者：
Webster LM
中科院分区：
文献类型：
--
作者：
Webster LM
宿主对寄生虫感染反应中涉及的基因的鉴定，既提供了一种了解免疫防御途径的方法，也提供了检查宿主与寄生虫共同进化的目标。大多数研究依赖于从模型系统衍生的候选基因方法来识别感兴趣的基因靶标，并且缺乏旨在提供自然群体免疫反应的整体概述的研究。我们在红松鸡（Lagopus lagopus scoticus）的自然种群中进行了一项实验，以控制细毛圆线虫寄生虫感染的水平。通过标准 cDNA 和由肠道、肝脏和脾脏产生的抑制消减杂交 (SSH) 文库检查个体的转录组反应，这些文库富集了对 T 反应表达的基因。 细小感染。从 cDNA 和 SSH 文库中分别鉴定出总共 2209 个和 3716 个独特的转录本序列。其中 45 个具有与免疫反应相关的基因本体注释。其中一些基因先前已在模型物种的实验室研究中报道过，对于胃肠道寄生虫感染的免疫反应很重要；然而，还发现了多个新基因。这些可能揭示松鸡对T的宿主反应所涉及的新途径。 tenuisand 提供了可用作其他物种候选基因的资源。
The identification of genes involved in a host’s response to parasite infection provides both a means for understanding the pathways involved in immune defence and a target for examining host–parasite co‐evolution. Most studies rely on a candidate gene approach derived from model systems to identify gene targets of interest, and there have been a dearth of studies geared towards providing a holistic overview of immune response from natural populations. We carried out an experiment in a natural population of red grouse (Lagopus lagopus scoticus) to manipulate levels ofTrichostrongylus tenuisparasite infection. The transcriptomic response of individuals was examined from standard cDNA and suppressive subtractive hybridization (SSH) libraries produced from gut, liver and spleen, enriching for genes expressed in response toT. tenuisinfection. A total of 2209 and 3716 unique transcript sequences were identified from the cDNA and SSH libraries, respectively. Forty‐five of these had Gene Ontology annotation associated with immune response. Some of these genes have previously been reported from laboratory‐based studies of model species as important in immune response to gastrointestinal parasite infection; however, multiple novel genes were also identified. These may reveal novel pathways involved in the host response of grouse toT. tenuisand provide a resource that can be utilized as candidate genes in other species.