Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes
Prc1-rich kinetochores are required for error-free acentrosomal spindle bipolarization during meiosis I in mouse oocytes
复制标题
小鼠卵母细胞减数分裂 I 期间无差错的中心体纺锤体双极化需要富含 Prc1 的着丝粒
DOI：
10.1038/s41467-020-16488-y
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发表时间：
2020
影响因子：
16.6
通讯作者：
Mary Herbert & Tomoya S. Kitajima
中科院分区：
文献类型：
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作者：
Shuhei Yoshida;Sui Nishiyama;Lisa Lister;Shu Hashimoto;Tappei Mishina;Aurélien Courtois;Hirohisa Kyogoku;Takaya Abe;Aki Shiraishi;Meenakshi Choudhary;Yoshiharu Nakaoka;Mary Herbert & Tomoya S. Kitajima
卵母细胞中的无中心体减数分裂代表了配子发生的挑战，需要纺锤体双极化而没有预定义的双极线索。虽然很多人知道的结构，促进acentrosomal微管成核，少有人知道的结构，介导纺锤体双极化哺乳动物卵母细胞。在这里，我们表明，在小鼠卵母细胞中，着丝粒需要纺锤体双极化在减数分裂I。这个过程是促进卵母细胞特异性，微管独立的富集的反平行微管交联剂Prc1在着丝粒通过Ndc80复合物。相反，在减数分裂II，细胞质中含有上调的因素，包括Prc1支持着丝粒独立的纺锤体双极化途径。纺锤体双极化依赖于着丝粒的模式是减数分裂I所必需的，以防止染色体分离错误。人类卵母细胞，纺锤体双极化据报道是容易出错的，表现出不可检测的着丝粒富集Prc1。这项研究揭示了小鼠无中心体纺锤体双极化中动粒的卵母细胞特异性功能，并为人类卵母细胞的易错性提供了见解。
Acentrosomal meiosis in oocytes represents a gametogenic challenge, requiring spindle bipolarization without predefined bipolar cues. While much is known about the structures that promote acentrosomal microtubule nucleation, less is known about the structures that mediate spindle bipolarization in mammalian oocytes. Here, we show that in mouse oocytes, kinetochores are required for spindle bipolarization in meiosis I. This process is promoted by oocyte-specific, microtubule-independent enrichment of the antiparallel microtubule crosslinker Prc1 at kinetochores via the Ndc80 complex. In contrast, in meiosis II, cytoplasm that contains upregulated factors including Prc1 supports kinetochore-independent pathways for spindle bipolarization. The kinetochore-dependent mode of spindle bipolarization is required for meiosis I to prevent chromosome segregation errors. Human oocytes, where spindle bipolarization is reportedly error prone, exhibit no detectable kinetochore enrichment of Prc1. This study reveals an oocyte-specific function of kinetochores in acentrosomal spindle bipolarization in mice, and provides insights into the error-prone nature of human oocytes.