Inhibition of allergen-induced airway remodeling by neonatal bacillus Calmette-Guerin vaccination is associated with interferon-gamma-producing T cells but not regulatory T cells in mice
Inhibition of allergen-induced airway remodeling by neonatal bacillus Calmette-Guerin vaccination is associated with interferon-gamma-producing T cells but not regulatory T cells in mice
复制标题
新生儿卡介苗疫苗接种对过敏原诱导的气道重塑的抑制作用与小鼠中产生干扰素 γ 的 T 细胞有关，但与调节性 T 细胞无关。
DOI：
10.1016/j.anai.2011.05.018
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发表时间：
2011-08-01
影响因子：
5.9
通讯作者：
Shen, Huahao
中科院分区：
文献类型：
--
作者：
Wang, Pingli;Zhang, Gensheng;Shen, Huahao
背景资料：对患者的流行病学评估和使用动物模型的研究表明，在生命早期接触牛分枝杆菌卡介苗（BCG）疫苗可预防哮喘的发生。目的：探讨新生儿多次接种卡介苗（BCG）对小鼠气道重塑及肺T细胞反应的影响。在5周龄和7周龄时，将小鼠致敏，然后用雾化卵清蛋白（OVA）每周3次连续8周进行激发。气道重塑和诱导肺T细胞responses.Results的程度进行了表征：新生儿多卡介苗接种减少了OVA诱导的重塑事件，包括支气管周围α-平滑肌肌动蛋白，支气管周围纤维化，气道上皮粘蛋白积累的水平。BCG疫苗接种还降低了支气管周围细胞转化生长因子β 1（TGF-β 1）的表达。相比之下，卡介苗接种增加了频率的干扰素-γ（IFN-γ）-产生的T细胞在肺和IFN-γ水平在BALF中，与肺调节性T细胞和IL-17-产生的T细胞没有影响。结论：我们的数据表明，多个卡介苗接种的新生儿减少过敏原诱导的气道重塑的指标特征。更重要的是，这种减少与肺调节性T细胞数量的增加无关，而是与产生IFN-γ的T细胞的增加相关。安过敏性哮喘免疫学2011;107：163-170。
Background: Epidemiological assessments of patients and studies using animal models show that exposure to Mycobacterium bovis bacillus Calmette-Guerin (BCG) vaccine in early life prevents asthma development. However, little is known about the potential of neonatal BCG vaccination in preventing the development of airway remodeling of asthma.Objective: To investigate the effects of multiple BCG vaccinations of neonates on the airway remodeling in mice and the accompanied pulmonary T cell responses.Methods: BALB/c neonates were vaccinated with BCG 3 times. At 5 and 7 weeks of age, the mice were sensitized and then challenged with aerosolized ovalbumin (OVA) 3 times per week for 8 successive weeks. The extent of airway remodeling and induced pulmonary T cell responses were characterized.Results: Multiple BCG vaccinations of neonates reduced OVA-induced remodeling events, including levels of peribronchial alpha-smooth muscle actin, peribronchial fibrosis, and airway epithelial mucin accumulation. The BCG vaccinations also decreased peribronchial cells expression of transforming growth factor beta 1 (TGF-beta 1). In contrast, BCG vaccinations increased the frequency of interferon-gamma (IFN-gamma)-producing T cells in the lung and IFN-gamma level in BALF, with no effects on pulmonary regulatory T cells and IL-17-producing T cells.Conclusions: Our data suggest that multiple BCG vaccinations of neonates reduced metrics characteristic of allergen-induced airway remodeling. More importantly, this decrease was not associated with an increased number of pulmonary regulatory T cells but instead correlated with an increase of IFN-gamma-producing T cells. Ann Allergy Asthma Immunol. 2011;107:163-170.