Induction of IgG antibodies by an anti-idiotype antibody mimicking disialoganglioside GD2.
Induction of IgG antibodies by an anti-idiotype antibody mimicking disialoganglioside GD2.
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通过模拟二唾液酸神经节苷脂 GD2 的抗独特型抗体诱导 IgG 抗体。
DOI：
10.1097/00002371-199801000-00010
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Bhattacharya-Chatterjee,MB
中科院分区：
文献类型：
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作者：
Sen,G;Chakraborty,M;Foon,KA;Reisfeld,RA;Bhattacharya-Chatterjee,MB
抗独特型（Id）单克隆抗体（mAb）1A 7免疫球蛋白Gl（IgGl，K），在同基因小鼠中针对鼠抗神经节苷脂GD 2 mAb 14 G2 a产生，模拟GD 2上的碳水化合物表位，并用作该二唾液酸神经节苷脂的替代蛋白抗原。用1A 7免疫同种异体C57 BL/6小鼠和兔诱导IgG同种型的抗GD 2抗体，其通过酶联免疫吸附测定（ELISA）识别纯化的GD 2，并通过荧光激活细胞分选仪（FACS）分析识别GD 2阳性人黑素瘤细胞（M21/P6）。斑点杂交分析进一步证实了抗血清对GD 2的特异性。这些抗血清还在抗体依赖性细胞毒性测定中特异性裂解GD 2阳性M21/P6靶细胞。综上所述，这些结果表明，抗Id 1A 7可以诱导GD 2特异性IgG抗体，其可以识别细胞表面相关的以及可溶性的二唾液酸神经节苷脂GD 2。
The anti-idiotype (Id) monoclonal antibody (mAb) 1A7 immunoglobulin Gl (IgGl, K), raised in syngeneic mice against the murine anti-ganglioside GD2 mAb 14G2a mimics a carbohydrate epitope on GD2 and serves as a surrogate protein antigen for this disialoganglioside. Immunization of allogeneic C57BL/6 mice and rabbits with 1A7 induced anti-GD2 antibodies of IgG isotype that recognize purified GD2 by enzyme-linked immunosorbent assay (ELISA) and GD2-positive human melanoma cells (M21/P6) by fluorescence-activated cell sorter (FACS) analysis. The specificity of the antisera for GD2 was further confirmed by dot-blot analysis. These antisera also specifically lyse GD2-positive M2l/P6 target cells in an antibodydependent cellular cytotoxicity assay. Taken together, these results suggest that the anti-Id 1A7 can induce GD2-specific IgG antibodies that can recognize cell surfaceassociated as well as soluble disialoganglioside GD2.