Lnc5926 is essential for early embryonic development in goats through regulation of ZSCAN4 and EIF1AX.
Lnc5926 is essential for early embryonic development in goats through regulation of ZSCAN4 and EIF1AX.
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Lnc5926 通过调节 ZSCAN4 和 EIF1AX 对于山羊早期胚胎发育至关重要。
DOI：
10.1016/j.theriogenology.2021.12.020
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发表时间：
2021-12
期刊：
影响因子：
2.8
通讯作者：
Liu Jun
中科院分区：
文献类型：
--
作者：
Zhang Qing;Li Wenjing;Feng Pei;Liu Yayi;Meng Peng;Chu Bo;Zhao Jianglin;Li Yanxue;Zhang Yong;Liu Jun
长链非编码RNA（longnoncodingRNA，lncRNA）在哺乳动物基因组中大量存在，并在许多生物学事件中发挥重要作用。然而，lncRNA调控胚胎发育的机制仍有待充分阐明。在此，我们通过在山羊早期胚胎中的敲低和过表达实验来研究lncRNA TCONS_00135926（简称lnc5926）的功能。Lnc5926在8细胞胚胎期的表达量显著高于其他阶段，这与胚胎基因组激活（EGA）基因的表达模式一致。在8细胞胚胎中，lnc5926基因敲除后的囊胚率显著低于对照组（0.2% vs.17.1%，p < 0.05），而卵裂率不受影响（71.9% vs.75.1%，p <0.05）。在胚胎中敲低或过表达lnc5926后，我们测量了潜在靶基因STAM、HACD 1、UBL 5、MIOX、ELF 1和关键EGA基因ZSCAN4和EIF1AX的表达水平。只有ZSCAN4和EIF1AX在lnc5926敲低后显著下调，并且这种作用被lnc5926过表达逆转。我们的结论是，lnc5926起着至关重要的作用，在山羊早期胚胎发育过程中，通过调节EGA相关基因的表达。
Long noncoding RNAs (lncRNAs) are abundant in mammalian genomes and have been found to play important roles in many biological events. However, the mechanism by which lncRNAs regulate embryonic development remains to be fully elucidated. Here, we investigated the function of the lncRNA, TCONS_00135926 (referred to as lnc5926), through knockdown and overexpression experiments in goat early embryos. Lnc5926 expression at the eight-cell embryonic stage was significantly higher than that at other stages, which was consistent with the pattern of embryonic genome activation (EGA) gene expression. The blastocyst rate after lnc5926 knockdown in eight-cell embryos was significantly lower than that in the control group (0.2% vs. 17.1%, p < 0.05), whereas the cleavage rate was not affected (71.9% vs. 75.1%, p ˃ 0.05). After knockdown or overexpression of lnc5926 in embryos, we measured expression levels of the potential target genes, STAM, HACD1, UBL5, MIOX, ELF1, and the key EGA genes, ZSCAN4 and EIF1AX. Only ZSCAN4 and EIF1AX were significantly downregulated after lnc5926 knockdown, and this effect was reversed by lnc5926 overexpression. We conclude that lnc5926 plays an essential role in early embryonic development in goats by regulating expression of EGA-associated genes.
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DOI：
10.1242/dev.083279
发表时间：
2012-12
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Vogt EJ;Meglicki M;Hartung KI;Borsuk E;Behr R
通讯作者：
Behr R
影响因子：
4.5
作者：
Yue M;Ogawa A;Yamada N;Charles Richard JL;Barski A;Ogawa Y
通讯作者：
Ogawa Y
影响因子：
64.5
作者：
Percharde M;Lin CJ;Yin Y;Guan J;Peixoto GA;Bulut-Karslioglu A;Biechele S;Huang B;Shen X;Ramalho-Santos M
通讯作者：
Ramalho-Santos M
DOI：
10.1242/dev.116996
发表时间：
2015-03-01
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Hamazaki N;Uesaka M;Nakashima K;Agata K;Imamura T
通讯作者：
Imamura T
影响因子：
1.9
作者：
Magnani, Luca;Johnson, Christine M.;Cabot, Ryan A.
通讯作者：
Cabot, Ryan A.