Enhanced bone regeneration via local low-dose delivery of PTH(1-34) in a composite hydrogel.
Enhanced bone regeneration via local low-dose delivery of PTH(1-34) in a composite hydrogel.
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DOI：
10.3389/fbioe.2023.1209752
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发表时间：
2023
影响因子：
5.7
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中科院分区：
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在支架材料中引入骨再生促进因子以提高骨诱导性能在骨组织工程和再生医学领域至关重要。本研究旨在开发一种具有高生物相容性的负载Sr-HA/PTH1-34的复合水凝胶系统。选择能够促进骨再生的特立帕肽(PTH1-34)作为生物活性因子。系统中引入锶取代羟基磷灰石（Sr-HA）来吸附PTH1-34，以促进生物活性并延迟释放周期。然后将负载PTH1-34的Sr-HA与水凝胶前驱体溶液混合，制备出具有良好生物相容性和高机械强度的复合水凝胶作为骨修复材料。实验表明Sr-HA吸附PTH1-34并实现PTH1-34的缓慢有效释放。体外生物学实验表明，负载Sr-HA/PTH1-34的水凝胶系统具有较高的生物相容性，允许细胞在表面良好生长。碱性磷酸酶活性和成骨基因表达的测量表明该复合系统可以促进MC3T3-E1细胞向成骨细胞分化。此外，体内颅骨缺损修复实验证实，这种复合水凝胶可以促进新骨的再生。综上所述，Sr-HA/PTH1-34复合水凝胶是一种极具前景的骨修复材料。
Introducing bone regeneration–promoting factors into scaffold materials to improve the bone induction property is crucial in the fields of bone tissue engineering and regenerative medicine. This study aimed to develop a Sr-HA/PTH1-34-loaded composite hydrogel system with high biocompatibility. Teriparatide (PTH1-34) capable of promoting bone regeneration was selected as the bioactive factor. Strontium-substituted hydroxyapatite (Sr-HA) was introduced into the system to absorb PTH1-34 to promote the bioactivity and delay the release cycle. PTH1-34-loaded Sr-HA was then mixed with the precursor solution of the hydrogel to prepare the composite hydrogel as bone-repairing material with good biocompatibility and high mechanical strength. The experiments showed that Sr-HA absorbed PTH1-34 and achieved the slow and effective release of PTH1-34. In vitro biological experiments showed that the Sr-HA/PTH1-34-loaded hydrogel system had high biocompatibility, allowing the good growth of cells on the surface. The measurement of alkaline phosphatase activity and osteogenesis gene expression demonstrated that this composite system could promote the differentiation of MC3T3-E1 cells into osteoblasts. In addition, the in vivo cranial bone defect repair experiment confirmed that this composite hydrogel could promote the regeneration of new bones. In summary, Sr-HA/PTH1-34 composite hydrogel is a highly promising bone repair material.