Adaptation of IFN-gamma ELISA and ELISPOT tests for feline tuberculosis
Adaptation of IFN-gamma ELISA and ELISPOT tests for feline tuberculosis
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DOI：
10.1016/j.vetimm.2008.04.006
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发表时间：
2008-08-15
影响因子：
1.8
通讯作者：
Jahans, Keith
中科院分区：
文献类型：
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作者：
Rhodes, Shelley G.;Gruffydd-Jones, Tim;Jahans, Keith
目前还没有可靠的猫结核病免疫诊断测试。英国家猫的感染被认为是通过接触相关的感染源而发生的，例如，牛和獾的牛分枝杆菌，啮齿类动物的田鼠分枝杆菌。在非洲国家公园，牛科动物的牛支原体感染问题日益严重，大型猫科动物通过摄入受感染的尸体进行传播。我们采用了猫科动物 ELISA 和 ELISPOT 检测方法，有可能提供第一个基于细胞的诊断测试，用于检测猫的结核病。我们测试了 18 只疑似分枝杆菌感染的猫的外周血单核细胞抗原特异性 IFN-γ 反应，将其活检材料共同提交给兽医实验室机构进行分枝杆菌培养和鉴定。十七只猫进行了 ELISA 测试，七只猫进行了 ELISPOT 测试（六只猫同时进行了 ELISA 和 ELISPOT 测试）。六只健康的对照猫为这些测试提供了基线数据。测量了对牛和禽结核菌素（PPDB 和 PPDA）以及 ESAT6 和 CFP10 蛋白质混合物的反应，以及阳性有丝分裂原（PMA 和钙离子载体）和阴性（中等）对照。总体而言，ELISPOT 和 ELISA 检测均被发现适合快速生成结果（分别为 2 次和 4 次），为牛分枝杆菌和田鼠分枝杆菌感染提供了良好的预测信息，但无法可靠地辨别鸟分枝杆菌感染。Crown 版权所有 (C) 2008 由 Elsevier B.V 出版。保留所有权利。
There are currently no reliable immunodiagnostic tests for feline tuberculosis. Infection of domestic cats in the UK is thought to Occur via their contact with the relevant reservoir of infection, e.g. cattle and badgers for Mycobacterium bovis, and rodents for M. microti. In the African National Parks, where M. bovis infection of Bovidae is an increasing problem, transmission to big cats is occurring via their ingestion of infected carcasses, We have adapted feline ELISA and ELISPOT assays to potentially provide the first cell-based diagnostic test for the detection of tuberculosis in cats. We tested peripheral blood mononuclear cell antigen-specific IFN-gamma responses of 18 cats Suspected of mycobacterial infection for which biopsy material was co-submitted to the Veterinary Laboratories Agency for mycobacterial culture and identification. Seventeen cats were tested by ELISA while seven cats were tested by ELISPOT (six cats were tested by both ELISA and ELISPOT). Six healthy control cats provided baseline data for these tests. Responses to bovine and avian tuberculins (PPDB and PPDA) and a protein cocktail of ESAT6 and CFP10 were measured, together with positive mitogen (PMA and calcium ionophore) and negative (medium) controls. Overall, both ELISPOT and ELISA tests were found to be Suitable For generating rapid results (2 and 4 (lays, respectively), which provided good predictive information for M. bovis and M. microti infections, but were unable to reliably discern M. avium infection. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.