The effects of stress hormones on growth of selected periodontitis related bacteria
The effects of stress hormones on growth of selected periodontitis related bacteria
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DOI：
10.1016/j.anaerobe.2013.09.001
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发表时间：
2013-12-01
期刊：
影响因子：
2.3
通讯作者：
Krueger, Monika
中科院分区：
文献类型：
--
作者：
Jentsch, H. F. R.;Maerz, Diana;Krueger, Monika
本研究的重点是在体外检查应激激素（儿茶酚胺：肾上腺素、去甲肾上腺素、多巴胺和氢化可的松：皮质醇）对牙周炎相关细菌的四种厌氧菌（具核梭杆菌、牙龈卟啉单胞菌、中间普雷沃菌和连翘坦纳菌）和一种兼性厌氧菌生长的影响。 （Eikenella corrodens）。通过两种不同的方法测定细菌生长：荧光原位杂交（FISH）和通过培养法进行活菌计数。为了模拟应激，每个单一菌株都在 37 摄氏度的厌氧室中，在含有三种不同浓度的每种激素的特殊生长培养基中生长。在所有应激激素存在下，具核球菌的生长都会增加。牙龈卟啉单胞菌的生长不受任何激素的显着影响。几乎所有测试的应激激素都抑制中间 P. intermedia 和 E. Corrodens 的生长。两种分析方法都表明，最高浓度的去甲肾上腺素和皮质醇促进了连翘的生长。不同的激素对体外牙周炎相关细菌的生长有不同的影响。看来细菌活力比细菌代谢活动受到的影响更大。几种应激激素会增加具核球菌的生长，特别是连翘的部分生长，并且可能对牙周病产生额外的负面影响。 (C) 2013 年，爱思唯尔有限公司出版。
The focus of this study was to examine in vitro the effects of stress hormones (catecholamines: epinephrine, norepinephrine, dopamine and hydrocortisone: cortisol) on the growth of four anaerobic species of periodontitis-related bacteria (Fusobacterium nucleatum, Porphyromonas gingivalis, Prevotella intermedia and Tannerella forsythia) and one facultative anaerobic species (Eikenella corrodens).Bacterial growth was determined by two different methods: fluorescence in situ hybridization (FISH), and the viable count by culture method. To simulate stress, each single strain was grown in a special growth medium with three different concentrations of each hormone, using an anaerobic chamber at 37 degrees C.Growth of E nucleatum increased in the presence of all stress hormones. Growth of P. gingivalis was not significantly influenced by any hormone. Growth of P. intermedia and E. corrodens was inhibited by almost all stress hormones tested. Both methods of analysis revealed that the highest concentrations of norepinephrine and cortisol increased the growth of T forsythia. Different hormones have a different effect on the growth of periodontitis-related bacteria in vitro. It appears that bacterial viability is more strongly influenced than is bacterial metabolic activity. The growth of E nucleatum particularly and partially of T. forsythia is increased by several stress hormones and may have an additional negative impact on periodontal disease. (C) 2013 Published by Elsevier Ltd.