How does Hsp90 function in RNAi-dependent heterochromatin assembly?
How does Hsp90 function in RNAi-dependent heterochromatin assembly?
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DOI：
10.1007/s00294-018-0866-0
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发表时间：
2018-07
期刊：
影响因子：
2.5
通讯作者：
Hiroaki Kato;K. Okazaki;T. Urano
中科院分区：
文献类型：
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作者：
Hiroaki Kato;K. Okazaki;T. Urano
热休克蛋白90（Heat-shock protein 90，Hsp 90）是近年来在裂殖酵母裂殖酵母中发现的一种RNA干扰（RNA interference，RNAi）依赖的异染色质组装沉默因子。由于已知Hsp 90有助于形成含小RNA的效应复合物，因此预期Hsp 90也参与裂殖酵母中的RNAi途径。然而，经过研究，我们发现很难确定Hsp 90如何调节细胞中RNAi依赖的异染色质组装。在hsp 90突变体细胞中缺乏可检测的小干扰RNA使我们无法检测hsp 90在siRNA加载细胞中的作用。此外，缺失编码Hsp 90共分子伴侣的基因似乎不影响RNAi依赖的近着丝粒沉默。阐明Hsp 90在RNAi依赖性异染色质组装中的作用的一种可能的方法是使用正向遗传筛选来鉴定将Hsp 90与其他已知RNAi因子连接的新因子。在这里，我们讨论了进行进一步筛查的好处，并提出了一些技术提示，以帮助确定新的因素。
Heat-shock protein 90 (Hsp90) was recently identified as a silencing factor required for RNA interference (RNAi)-dependent heterochromatin assembly in the fission yeastSchizosaccharomyces pombe. As Hsp90 is known to contribute to the formation of small RNA-containing effector complexes, it would be expected that Hsp90 is also involved in the RNAi pathway in fission yeast. However, upon investigation, we found it very difficult to determine how Hsp90 modulates RNAi-dependent heterochromatin assembly in the cell. A lack of detectable small interfering RNAs inhsp90mutant cells prevented us from examining the role of Hsp90 in the siRNA loading in the cell. In addition, deletion of genes encoding co-chaperones for Hsp90 appears not to affect RNAi-dependent pericentromeric silencing. One possible approach for elucidating the role of Hsp90 in RNAi-dependent heterochromatin assembly is the use of forward genetic screens to identify novel factors linking Hsp90 with other known RNAi factors. Here, we discuss the benefits of conducting further screenings and present some technical hints to help identify new factors.