Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress.
Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress.
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DOI：
10.1111/j.0031-9317.2004.00294.x
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发表时间：
2004-05
影响因子：
6.4
通讯作者：
A. Sofo;B. Dichio;C. Xiloyannis;A. Masia
中科院分区：
文献类型：
--
作者：
A. Sofo;B. Dichio;C. Xiloyannis;A. Masia
橄榄（Olea europaea L.）它通常生长在地中海盆地，能够抵抗严重和长期的干旱。以2年生橄榄为试材，测定了脯氨酸（PRO）、丙二醛（MDA）含量和脂氧合酶（LOX）活性。'Coratina'）在以高温和高光合光子通量密度水平为特征的环境条件下生长，并逐渐经受受控的水分亏缺20天。在实验之前和实验期间，收集叶和根样品并分析PRO和MDA。叶片和根系中PRO的含量随着干旱胁迫程度的加重而增加。随着干旱胁迫程度的增加，叶片和根组织中LOX活性和MDA含量也显著增加。在试验期间测定了蒸腾速率、光合速率、气孔导度和气孔下CO2浓度。PRO的积累表明PRO在耐旱性中可能起作用。MDA含量和LOX活性的增加表明水分亏缺与膜脂过氧化机制有关。
The olive tree (Olea europaea L.) is commonly grown in the Mediterranean basin and is able to resist severe and prolonged drought. Levels of proline (PRO) and malondialdehyde (MDA), and the lipoxygenase (LOX) activity were determined in 2-year-old olive plants (cv. 'Coratina') grown in environmental conditions characterized by high temperatures and high photosynthetic photon flux density levels and gradually subjected to a controlled water deficit for 20 days. Before and during the experimental period, leaf and root samples were collected and analysed for PRO and MDA. The levels of PRO increased in parallel with the severity of drought stress in both leaves and roots. Significant increases of LOX activity and MDA content were also observed during the progressive increment of drought stress in both leaf and root tissues. Measurements of transpiration and photosynthetic rate, stomatal conductance and substomatal CO(2) concentration were carried out during the experiment. The accumulation of PRO indicates a possible role of PRO in drought tolerance. The increases of MDA content and LOX activity show that the water deficit is associated with lipid peroxidation mechanisms.