The BRET technology and its application to screening assays.
The BRET technology and its application to screening assays.
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DOI：
10.1002/biot.200700222
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发表时间：
2008-03-01
影响因子：
4.7
通讯作者：
Couturier, Cyril
中科院分区：
文献类型：
--
作者：
Bacart, Johan;Corbel, Caroline;Couturier, Cyril
生物发光共振能量转移（BRET）方法是基于发光酶和荧光受体之间的共振能量转移。自1999年首次描述以来，已经描述了使用不同衬底和能量供体/受体对的BRET的几个版本。今天，BRET被认为是研究活细胞中蛋白质-蛋白质相互作用动力学的最通用技术之一。各种研究已经应用基于BRET的测定来筛选新的疾病相关蛋白酶的受体配体和抑制剂。蛋白质-蛋白质相互作用抑制剂有可能成为一类新的主要治疗药物，BRET技术有望在此类化合物的鉴定中发挥重要作用。这篇综述介绍了原来的BRET为基础的方法，最近的变种，和潜在的应用，药物筛选。
The bioluminescence resonance energy transfer (BRET) method is based on resonance energy transfer between a light-emitting enzyme and a fluorescent acceptor. Since its first description in 1999, several versions of BRET have been described using different substrates and energy donor/acceptor couples. Today, BRET is considered as one of the most versatile techniques for studying the dynamics of protein-protein interactions in living cells. Various studies have applied BRET-based assays to screen new receptor ligands and inhibitors of disease-related-proteases. Inhibitors of protein-protein interactions are likely to become a new major class of therapeutic drugs, and BRET technology is expected to play an important role in the identification of such compounds. This review describes the original BRET-based methodology, more recent variants, and potential applications to drug screening.