The response and detoxification strategies of three freshwater phytoplankton species, Aphanizomenon flos-aquae, Pediastrum simplex, and Synedra acus, to cadmium
The response and detoxification strategies of three freshwater phytoplankton species, Aphanizomenon flos-aquae, Pediastrum simplex, and Synedra acus, to cadmium
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水花束丝藻、单纯盘星藻和水丝藻三种淡水浮游植物对镉的响应和解毒策略
DOI：
10.1007/s11356-015-5161-8
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发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Zhongxing Wu
中科院分区：
文献类型：
--
作者：
Yanjun Yang;Jinzhu Xu;Junqiong Shi;Zhongxing Wu
研究了3种淡水浮游植物对Cd的响应和解毒机制，包括蓝藻Aphanizomenon Flos-Aquae、绿藻Pedistar Singex和硅藻Synedra acus。每种藻类的细胞生长在10d以上，暴露96h后，测定了叶绿素a荧光、Cd的生物积累(包括表面吸附Cd和胞内Cd)和植物螯合素(PC)的合成。当Cd浓度为≥5 mg L(-1)时，3种浮游植物的生长受到明显抑制。与单纯假单胞菌相比，尖吻假单胞菌和水华假单胞菌的生长抑制作用更强。叶绿素荧光参数，包括PSII的最大量子效率(Fv/Fm)和J点的相对可变荧光(Vj)的变化表明，Cd浓度的增加损害了三种植物的PSII。暴露96h后，随着培养液中Cd浓度的增加，三种藻细胞表面吸附Cd和胞内Cd的积累量均显著增加，10 mg L(-1)处理后水华、赤潮藻和刺参的Cd积累量分别是对照的245、658和1670倍。总硫醇的积累趋势与Cd的积累趋势相似。在所有Cd处理中，Pc3都存在于水曲柳和单叶黄曲霉中。谷胱甘肽(GSH)和PC2也是对高浓度Cd暴露的反应。只有在10 mg L(-1)Cd暴露浓度下，才能在尖吻海鞘中检出PC4。3种浮游植物的胞内Cd/PC比值均随Cd浓度的增加而增加，而单纯螺旋藻和尖吻假单胞藻的细胞内Cd/PC比值与生长抑制率呈线性关系。我们的结果表明，金属的解毒机制依赖于物种。这项研究表明，不同的金属解毒策略，如镉积累和PCS，可能解释了为什么藻类对不同水平的Cd具有不同的敏感性。
The response and detoxification mechanisms of three freshwater phytoplankton species (the cyanobacterium Aphanizomenon flos-aquae, the green alga Pediastrum simplex, and the diatom Synedra acus) to cadmium (Cd) were investigated. The cell growth of each species was measured over 10 days, and chlorophyll a fluorescence, Cd bioaccumulation (including surface-adsorbed and intracellular Cd), and phytochelatin (PC) synthesis were determined after 96-h exposures. The growth of the three phytoplankton species was significantly inhibited when Cd concentrations were ≥5 mg L(-1). Compared with P. simplex, greater growth inhibition in S. acus and A. flos-aquae occurred. The changes in chlorophyll fluorescence parameters including the maximal quantum yield of PSII (Fv/Fm) and relative variable fluorescence of the J point (Vj) demonstrated that the increase in Cd concentration damaged PSII in all three species. After 96-h exposures, the accumulation of surface-adsorbed Cd and intracellular Cd increased significantly in all three species, with the increase of Cd concentrations in the media; total cadmium accumulation was 245, 658, and 1670 times greater than that of the control in A. flos-aquae, P. simplex, and S. acus, respectively, after exposure to 10 mg L(-1). Total thiols exhibited a similar trend to that of Cd accumulation. PC3 was found in A. flos-aquae and P. simplex in all Cd treatments. Glutathione (GSH) and PC2 were also produced in response to exposure to high concentrations of Cd. PC4 was only discovered at exposure concentrations of 10 mg L(-1) Cd and only in S. acus. The intracellular Cd/PCs ratio increased in all three phytoplankton with an increase in Cd concentrations, and a linear relationship between the ratio and the growth inhibition rates was observed with P. simplex and S. acus. Our results have demonstrated that metal detoxification mechanisms were dependent on the species. This study suggested that the variance of metal detoxification strategies, such as cadmium accumulation and PCs, might be an explanation why algal species have different sensitivity to Cd at various levels.
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影响因子：
2.8
作者：
GEKELER, W;GRILL, E;ZENK, MH
通讯作者：
ZENK, MH
影响因子：
7.4
作者：
Cobbett, CS
通讯作者：
Cobbett, CS
DOI：
10.1039/c39910001129
发表时间：
1991
期刊：
Journal of The Chemical Society, Chemical Communications
影响因子：
--
作者：
H. Strasdeit;A. Duhme;R. Kneer;M. Zenk;C. Hermes;H. Nolting
通讯作者：
H. Strasdeit;A. Duhme;R. Kneer;M. Zenk;C. Hermes;H. Nolting
影响因子：
9.8
作者：
Sunda, WG;Huntsman, SA
通讯作者：
Huntsman, SA
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
Reto J Strassert;Alaka Srivastava 'and Govindjee
通讯作者：
Reto J Strassert;Alaka Srivastava 'and Govindjee