What experimental approaches (eg, in vivo, in vitro, tissue retrieval) are effective in investigating the biologic effects of particles?
What experimental approaches (eg, in vivo, in vitro, tissue retrieval) are effective in investigating the biologic effects of particles?
复制标题
DOI：
10.5435/00124635-200800001-00013
复制
发表时间：
2008-01-01
影响因子：
3.2
通讯作者：
O'Keefe, Regis
中科院分区：
文献类型：
--
作者：
Bostrom, Mathias;O'Keefe, Regis
了解导致假体周围骨质溶解的复杂细胞和组织机制和相互作用需要许多实验方法，每种方法都有其自身的优点和局限性。体外模型允许分离单个细胞群，并进一步加深了我们对颗粒-细胞相互作用的理解;然而，它们是有限的，因为它们不能模拟发生多细胞相互作用的复杂组织环境。体内动物模型研究与假体周围骨质溶解相关的组织相互作用，但物种的选择以及植入物系统是否承受机械载荷或未加载条件对于评估这些模型是否可外推至临床条件至关重要。对从骨质溶解临床病例中取出的组织进行严格分析，为研究骨质溶解的生物学过程提供了一种不同的方法，但其局限性在于所分析的组织代表了该过程的终末期，因此可能无法充分反映该过程。
Understanding the complex cellular and tissue mechanisms and interactions resulting in periprosthetic osteolysis requires a number of experimental approaches, each of which has its own set of advantages and limitations. In vitro models allow for the isolation of individual cell populations and have furthered our understanding of particle-cell interactions; however, they are limited because they do not mimic the complex tissue environment in which multiple cell interactions occur. In vivo animal models investigate the tissue interactions associated with periprosthetic osteolysis, but the choice of species and whether the implant system is subjected to mechanical load or to unloaded conditions are critical in assessing whether these models,can be extrapolated to the clinical condition. Rigid analysis of retrieved tissue from clinical cases of osteolysis offers a different approach to studying the biologic process of osteolysis, but it is limited in that the tissue analyzed represents the end-stage of this process and, thus, may not reflect this process adequately.