Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase
Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase
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两种新型抗氨酰 tRNA 合成酶抗体：半胱氨酰 tRNA 合成酶和缬氨酰 tRNA 合成酶自身抗体
DOI：
10.1016/j.autrev.2022.103204
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发表时间：
2022
期刊：
影响因子：
13.6
通讯作者：
Akiyama M.
中科院分区：
文献类型：
--
作者：
Muro Y;Yamashita Y;Koizumi H;Ogawa-Momohara M;Takeichi T;Mitsuma T;Akiyama M.
抗氨基酰基trna合成酶（抗ars）抗体对识别炎症性肌病患者的临床亚群是有用的。由于抗ars阳性患者的肌炎具有一系列独特的非肌病表现，包括间质性肺疾病、机械性手和关节痛，因此这些患者被归类为抗合成酶综合征。已鉴定出8种急性呼吸道综合征的自身抗体。在其他12种ars中，有8种是“OJ”多合成酶复合物的组分。在炎性肌病患者中未发现针对其余四种ars （cyars、ValARS、SerARS和TrpARS）的自身抗体。在这项研究中，我们首先通过我们建立的内部ELISA筛选了300多名日本皮肌炎（DM）患者的样本，以寻找针对上述四种ars的自身抗体。由于两名糖尿病患者的血清对CysARS或ValARS有特异性反应，我们通过免疫沉淀（IP）和细胞提取物的免疫印迹（IP - western blotting）测定了它们的反应性。一名患者具有抗合成酶综合征的几个特征，但另一名患者没有。各种抗ars抗体的临床差异有待在今后的工作中进一步探讨。
Anti-aminoacyl-tRNA synthetase (anti-ARS) antibodies are useful for identifying a clinical subset of patients with inflammatory myopathies. Since the myositis of anti-ARS-positive patients is characterized by a unique set of non-myopathic manifestations, including interstitial lung disease, mechanic's hands, and arthralgia, the patients are classified as having anti-synthetase syndrome. Autoantibodies have been identified to eight kinds of ARSs. Of the other 12 ARSs, eight are components of the “OJ” multi-synthetase complex. Autoantibodies to the four remaining ARSs (CysARS, ValARS, SerARS, and TrpARS) have not been reported to be present in patients with inflammatory myopathies.In this study, we first screened samples from more than 300 Japanese patients majorly consisting of those with dermatomyositis (DM) by our established in-house ELISA to find autoantibodies against the four ARSs described above. Since sera from two DM patients specifically reacted to CysARS or ValARS, we determined their reactivities by immunoprecipitation (IP) with the corresponding recombinant proteins and IP–Western blotting with cellular extract. One patient had several features found in anti-synthetase syndrome, but the other did not. The clinical differences among the various anti-ARS antibodies should be explored in a future work.