Characteristics of eight X-STR loci for forensic purposes in the Chinese population
Characteristics of eight X-STR loci for forensic purposes in the Chinese population
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DOI：
10.1007/s00414-009-0386-z
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发表时间：
2011-01-01
影响因子：
2.1
通讯作者：
Hou, Yi-ping
中科院分区：
文献类型：
--
作者：
Luo, Hai-bo;Ye, Yi;Hou, Yi-ping
X染色体短串联重复序列（ChrX STR）基因座是近年来应用于法医学实践的一个新的研究热点。由于ChrX基因独特的遗传特性，ChrX STR基因座间的重组和连锁不平衡在不同性别和不同群体间存在差异。然而，缺乏中国人群ChrX STR基因座重组和连锁不平衡分析的数据。本研究采用Mentype Argus X-8 PCR扩增试剂盒（DXS 10135-DXS 8378、DXS 7132-DXS 10074、HPRTB-DXS 10101和DXS 10134-DXS 7423）对303例中国汉族无关个体（男203例，女100例）进行了PCR分析。应用HapMap LD作图和ARLEQUIN 3.1软件分析8个ChrX STR基因座的重组和连锁不平衡。获得了8个位点的等位基因频率和群体法医学遗传学参数。我们的研究结果揭示了重组热点，并没有明显的证据，LD在中国人群中的8个位点。我们的工作表明，在中国人群中，单基因座频率而不是单倍型频率应该被应用于法医学实践。
X-chromosomal short tandem repeats (ChrX STRs) loci are used for forensic practice in recent years. Considering the unique heredity characteristics of ChrX, recombination and linkage disequilibrium (LD) among ChrX STR loci vary between male and female and different populations as well. However, there is a lack of data for analysis of recombination and linkage disequilibrium on ChrX STR loci in the Chinese population. In this work, a total of 303 unrelated individuals (203 males and 100 females) in the Chinese Han population were analyzed with Mentype Argus X-8 PCR amplification kit (DXS10135-DXS8378, DXS7132-DXS10074, HPRTB-DXS10101, and DXS10134-DXS7423). The recombination and linkage disequilibrium of the eight ChrX STR loci were investigated with HapMap LD plots and software ARLEQUIN 3.1. Allele frequencies of the eight loci and further population forensic genetic parameters were obtained. Our results revealed hotspots for recombination, and there was no obvious evidence for LD among the eight loci in the Chinese population. Our work implied that single locus frequencies rather than haplotype frequencies should be applied for forensic practice in the Chinese population.