Human p150,95 alpha-subunit: genomic organization and analysis of the 5'-flanking region.
Human p150,95 alpha-subunit: genomic organization and analysis of the 5'-flanking region.
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人类 p150,95 α 亚基：基因组组织和 5 侧翼区域分析。
DOI：
10.1089/dna.1992.11.123
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发表时间：
1992
影响因子：
3.1
通讯作者：
Hall,RE
中科院分区：
文献类型：
--
作者：
Noti,JD;Gordon,M;Hall,RE
LFA-1，Mac-1，和p150，95包括一个家庭的细胞表面糖蛋白介导的粘附相互作用的骨髓和淋巴细胞。这些糖蛋白是由共同的β亚基和不同的α亚基组成的异二聚体。分离p150，95 α亚基的染色体基因，以提供研究该基因表达机制的框架。该基因跨越30 kb的DNA，包含31个外显子。与Corbiet等人先前的报告一致。（1990），发现外显子被分成由大内含子分隔的五组。胞外结构域在外显子2至30中编码，而跨膜和胞质结构域在外显子30和31中编码。我们以多种方式扩展了这些发现。第一个外显子含有5′非翻译区。5′侧翼序列长2，163 bp，包含第一个内含子和几个转录起始位点，前有两个TATA序列和两个GC样盒。各种DNA结合蛋白的其他序列基序是存在的，并进行了讨论。将细菌氯霉素乙酰转移酶基因（CAT）与约5.3kb的5′-侧翼DNA以及该区域的亚克隆片段融合，并转染人前单核细胞系U937。CAT的表达是可诱导的佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）和完全表达是依赖于内含子1的存在和序列上游的2,163-bp的侧翼DNA。此外，内含子1和更上游的区域含有功能性顺式作用序列。
LFA-1, Mac-1, and p150,95 comprise a family of cell-surface glycoproteins that mediate adhesive interactions of myeloid and lymphoid cells. These glycoproteins are heterodimers composed of a common β-subunit and distinct α-subunits. The chromosomal gene for the α-subunit of p150,95 was isolated to provide a framework from which to study the mechanisms for expression of the gene. The gene spans 30 kb of DNA and contains 31 exons. In agreement with a previous report by Corbiet al.(1990), the exons were found to be divided into five groups separated by large introns. The extracellular domains are encoded in exons 2 through 30 while the transmembrane and cytoplasmic domains are encoded in exons 30 and 31. We have expanded these findings in a number of ways. The first exon contains the 5′ untranslated region. The 2,163-bp 5′-flanking sequence contains the first intron and several putative transcriptional initiation sites preceded by two TATA sequences and two GC-like boxes. Additional sequence motifs for a variety of DNA-binding proteins are present and discussed. Fusions of the bacterial chloramphenicol acetyltransferase gene (CAT) to approximately 5.3 kb of 5′-flanking DNA and also to subcloned fragments of this region were constructed and transfected into the human promonocytic cell line, U937. CAT expression was inducible with phorbol-12-myristrate-13-acetate (PMA) and full expression was dependent on the presence of intron 1 and sequences upstream from the 2,163-bp flanking DNA. Additionally, intron 1 and a region further upstream contain functionalcis-acting sequences.