IDENTIFICATION OF NUCLEAR RECEPTORS FOR VIP ON A HUMAN COLONIC ADENOCARCINOMA CELL-LINE
IDENTIFICATION OF NUCLEAR RECEPTORS FOR VIP ON A HUMAN COLONIC ADENOCARCINOMA CELL-LINE
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DOI：
10.1126/science.2825352
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发表时间：
1987-12-11
期刊：
影响因子：
56.9
通讯作者：
KAGNOFF, MF
中科院分区：
文献类型：
--
作者：
OMARY, MB;KAGNOFF, MF
血管活性肠肽（VIP）是一种具有广泛组织分布的神经肽。尽管其确切功能尚不清楚，但人们认为它至少部分是通过与细胞表面受体相互作用来发挥其作用的。 VIP 的核受体现已通过 125I 标记的 VIP 与人结肠腺癌细胞系 (HT29) 细胞核的特异性结合以及 125I 标记的 VIP 与其完整细胞核上的受体交联来鉴定。相比之下，125I 标记的转铁蛋白仅显示出与细胞核的背景结合，但显示出与完整细胞的显着结合。通过电子显微镜进一步证实了分离的细胞核的纯度。 VIP 交联的核和细胞表面受体的表观分子大小相似但不相同。
Vasoactive intestinal peptide (VIP) is a neuropeptide with broad tissue distribution. Although its precise function is unknown, it is thought to exert its effects, at least in part, by interacting with cell surface receptors. Nuclear receptors for VIP have now been identified by specific binding of 125I-labeled VIP to nuclei of a human colonic adenocarcinoma cell line (HT29) and by cross-linking of 125I-labeled VIP to its receptor on intact nuclei. In contrast, 125I-labeled transferrin shows only background binding to nuclei but significant binding to intact cells. Purity of the isolated nuclei was further substantiated by electron microscopy. The apparent molecular sizes of the VIP-cross-linked nuclear and cell surface receptors are similar but not identical.