Solid-substrate fermentation of corn fiber by Phanerochaete chrysosporium and subsequent fermentation of hydrolysate into ethanol.
Solid-substrate fermentation of corn fiber by Phanerochaete chrysosporium and subsequent fermentation of hydrolysate into ethanol.
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DOI：
10.1021/jf0728404
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发表时间：
2008-05
影响因子：
6.1
通讯作者：
Prachand Shrestha;M. Rasmussen;Samir Kumar Khanal;A. Pometto;J. Leeuwen
中科院分区：
文献类型：
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作者：
Prachand Shrestha;M. Rasmussen;Samir Kumar Khanal;A. Pometto;J. Leeuwen
本研究的目的是开发一种利用玉米纤维生产乙醇的真菌工艺。使用 1 L 聚丙烯瓶中的白腐真菌 Phanerochaete chrysosporium 作为反应器，在 37 摄氏度下孵育长达 3 天，进行实验室规模的固体基质发酵。黄孢假单胞菌原位产生的胞外酶可降解木质素并增强玉米纤维中多糖的糖化作用。生物量重量损失百分比和 Klason 木质素减少百分比分别为 34% 和 41%。培养2天后在37℃厌氧培养减少了真菌的糖消耗并增强了原位纤维素分解酶的活性。玉米纤维与黄孢假单胞菌进行两天需氧固体培养基发酵，然后进行厌氧静态深层培养发酵，6 天后可产生 1.7 克乙醇/100 克玉米纤维，而酵母（酿酒酵母）与黄孢假单胞菌共培养可提高乙醇产量，每 100 克玉米纤维可生产 3 克乙醇。特定酶活性测定表明淀粉和半/纤维素对可发酵糖的贡献。
The goal of this study was to develop a fungal process for ethanol production from corn fiber. Laboratory-scale solid-substrate fermentation was performed using the white-rot fungus Phanerochaete chrysosporium in 1 L polypropylene bottles as reactors via incubation at 37 degrees C for up to 3 days. Extracellular enzymes produced in situ by P. chrysosporium degraded lignin and enhanced saccharification of polysaccharides in corn fiber. The percentage biomass weight loss and Klason lignin reduction were 34 and 41%, respectively. Anaerobic incubation at 37 degrees C following 2 day incubation reduced the fungal sugar consumption and enhanced the in situ cellulolytic enzyme activities. Two days of aerobic solid-substrate fermentation of corn fiber with P. chrysosporium, followed by anaerobic static submerged-culture fermentation resulted in 1.7 g of ethanol/100 g of corn fiber in 6 days, whereas yeast ( Saccharomyces cerevisiae) cocultured with P. chrysosporium demonstrated enhanced ethanol production of 3 g of ethanol/100 g of corn fiber. Specific enzyme activity assays suggested starch and hemi/cellulose contribution of fermentable sugar.