DAPI fluorescence in nuclei isolated from tumors
DAPI fluorescence in nuclei isolated from tumors
复制标题
DOI：
10.1369/jhc.4b6563.2005
复制
发表时间：
2005-08-01
影响因子：
3.2
通讯作者：
Dandekar, PD
中科院分区：
文献类型：
--
作者：
Krishan, A;Dandekar, PD
在用核分离介质-4，6-二脒基-2-苯基吲哚二盐酸盐（NIM-DAPI）染色的某些人实体瘤的DNA直方图中，G（0）/G（1）峰的变异系数（CV）较宽，在核体积与DNA散点图中，观察到显著的斜率。为了确定其原因，将来自冷冻乳腺肿瘤的细胞核用NIM-DAPI染色，并在PBS中稀释或重悬后进行分析。在双色（蓝色与红色）分析中，大部分斜率和宽CV是由于NIM-DAPI染色的细胞核的红色荧光，其在PBS中稀释或重悬时减少，导致斜率消除和CV收紧。
In DNA histograms of some human solid tumors stained with nuclear isolation medium-4,6-diamidino-2-phenylindole dihydrochloride (NIM-DAPI), the coefficient of variation (CV) of the G(0)/G(1) peak was broad, and in nuclear volume vs DNA scattergrams, a prominent slope was seen. To determine the cause for this, nuclei from frozen breast tumors were stained with NIM-DAPI and analyzed after dilution or resuspension in PBS. In two-color (blue vs red) analysis, most of the slope and broad CV was due to red fluorescence of nuclei stained with NIM-DAPI, which was reduced on dilution or resuspension in PBS, resulting in elimination of the slope and tightening of the CV.