Biomagnetic glass beads for protein separation and detection based on surface-enhanced Raman scattering
Biomagnetic glass beads for protein separation and detection based on surface-enhanced Raman scattering
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DOI：
10.1039/c2ay25244a
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发表时间：
2012-05
影响因子：
3.1
通讯作者：
Lei Chen;X. Han;Zhinan Guo;Xu Wang;Weidong Ruan;Wei Song;Bing Zhao;Y. Ozaki
中科院分区：
文献类型：
--
作者：
Lei Chen;X. Han;Zhinan Guo;Xu Wang;Weidong Ruan;Wei Song;Bing Zhao;Y. Ozaki
本文提出了一种基于表面增强拉曼散射（SERS）的磁性玻璃微珠蛋白检测方法。制备了二氧化硅包覆的磁性纳米颗粒（Fe3O4），并通过席夫反应进行醛基功能化，可捕获溶液中的蛋白质，与以往的磁性纳米颗粒和基于sers的检测方法相比，具有更好的生物相容性。具有特殊识别特征的靶蛋白和分子被磁性纳米颗粒捕获并分离，然后进行基于sers的检测。此外，这些生物磁性玻璃微珠和检测方案被发现在蛋白质免疫测定和蛋白质配体识别中有用，甚至在混合系统（两种蛋白质配体）。所提出的磁性纳米颗粒具有生物相容性，可通过外部磁力操纵，并且在银纳米颗粒染色后高度敏感，这使得该方案非常有希望用于高通量蛋白质分析。
A novel surface-enhanced Raman scattering (SERS)-based detection method for proteins attached to magnetic glass beads have been developed in this study. Silica coated magnetic nanoparticles (Fe3O4) are prepared and aldehyde-functionalized for capturing proteins in solutions by Schiff reaction, which is more biocompatible than previous magnetic nanoparticle and SERS-based detection methods. Target protein and molecule with a specially recognized feature are captured and separated by the magnetic nanoparticles, followed by SERS-based detection. Moreover, these biomagnetic glass beads and detection protocol are found to be useful in protein immunoassay and protein-ligand recognition even in a mixture system (two protein ligands). The proposed magnetic nanoparticles are biocompatible, manipulatable by an external magnetic force and highly sensitive after silver nanoparticles staining, which makes the protocol quite promising for high throughput protein assays.