Taura syndrome virus (TSV) lesion development and the disease cycle in the Pacific white shrimp Penaeus vannamei
Taura syndrome virus (TSV) lesion development and the disease cycle in the Pacific white shrimp Penaeus vannamei
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DOI：
10.3354/dao036081
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发表时间：
1999-05-12
影响因子：
1.4
通讯作者：
White, BM
中科院分区：
文献类型：
--
作者：
Hasson, KW;Lightner, DV;White, BM
通过组织学和基因探针分析实验感染特定病原体（SPF）的南美白对虾采样的时间间隔，桃拉综合征病毒（TSV）的疾病周期重新定义。该周期由3个重叠但临床和组织学上不同的阶段组成：类似于7天的急性期至急性期、类似于5天的过渡期（以前称为慢性期或恢复期）和确定的慢性期。急性期的特征是迅速发展为严重的、多灶性的至弥漫性的表皮上皮坏死和高死亡率。利用原位杂交分析，感染前裂解表皮上皮细胞显示非常强的TSV阳性探针信号，并共3个阶段的急性期坏死的描述。存活的凡纳滨对虾然后进入过渡期，其在组织学上通过表皮上皮区域内的多灶性黑化病变（消退急性期病变）、局灶性活性急性期病变和淋巴器官（LO）球状体发育的开始来区分。对过渡性感染虾的基因探针分析揭示了活动性急性期病变的探针阳性病灶、形态正常的LO小管壁内的弥漫性探针信号和/或发育中的LO球状体内的局灶性探针信号。在此阶段存活的虾在蜕皮后进入慢性期感染。慢性期的定义特征包括死亡停止、恢复正常行为模式、完全不存在可见的黑化病变和表皮上皮的急性期组织学病变，以及直接由许多LO球状体快速发育引起的显著LO肥大，其中一些球状体通过原位杂交分析为TSV阳性。
The Taura syndrome virus (TSV) disease cycle was redefined through histological and gene probe analysis of experimentally infected specific pathogen-free (SPF) Penaeus vannamei sampled at timed intervals. The cycle consists of 3 overlapping, but clinically and histologically distinct, phases: a similar to 7 d peracute to acute phase, a similar to 5 d transition phase (previously termed the chronic or recovery phase), and a definitive chronic phase. The acute phase is characterized by the rapid development of severe, multifocal to diffuse cuticular epithelial necrosis and high mortalities. Using in situ hybridization analysis, infected pre-lytic cuticular epithelial cells display very strong TSV-positive probe signals, and a total of 3 stages of acute phase necrosis are described. Surviving P, vannamei then enter the transition phase, which is distinguished histologically by multifocal melanized lesions within regions of the cuticular epithelium (resolving acute phase lesions), focal active acute phase lesions, and the onset of lymphoid organ (LO) spheroid development. Gene probe analysis of transitionally infected shrimp reveals probe-positive foci of active acute phase lesions, a diffuse probe signal within the walls of morphologically normal LO tubules and/or focal probe signals within developing LO spheroids. Shrimp surviving this stage enter the chronic phase infection after ecdysis. The defining characteristics of the chronic phase include the cessation of mortalities, the resumption of normal behavioral patterns, the complete absence of visible melanized lesions and acute phase histological lesions of the cuticular epithelium, and marked LO hypertrophy directly resulting from the rapid development of numerous LO spheroids, some of which are TSV positive by in situ hybridization analysis.