Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery
Implantation of dendritic cells in injured adult spinal cord results in activation of endogenous neural stem/progenitor cells leading to de novo neurogenesis and functional recovery
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DOI：
10.1002/jnr.20086
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发表时间：
2004-05-15
影响因子：
4.2
通讯作者：
Toda, M
中科院分区：
文献类型：
--
作者：
Mikami, Y;Okano, H;Toda, M
我们报告了一种治疗脊髓损伤的方法，包括植入树突状细胞（DC），树突状细胞在免疫系统中起抗原呈递细胞的作用。这种治疗的新机制产生功能恢复。在所测试的免疫细胞中，DC在体外显示出诱导神经干/祖细胞（NSPCs）增殖和存活的最强活性。此外，在DC植入的成年小鼠中，损伤脊髓中的内源性NSPCs被激活用于有丝分裂的新生神经发生。这些树突状细胞产生神经营养素-3并激活损伤脊髓中的内源性小胶质细胞。行为学分析显示，与对照小鼠相比，DC植入小鼠的运动功能显著恢复。我们的研究结果表明，DC植入发挥营养作用，包括激活内源性NSPCs，导致损伤的成年脊髓的修复。(C)2004 Wiley-Liss，Inc.
We report a treatment for spinal cord injury involving implantation of dendritic cells (DCs), which act as antigen-presenting cells in the immune system. The novel mechanisms underlying this treatment produce functional recovery. Among the immune cells tested, DCs showed the strongest activity inducing proliferation and survival of neural stem/progenitor cells (NSPCs) in vitro. Furthermore, in DC-implanted adult mice, endogenous NSPCs in the injured spinal cord were activated for mitotic de novo neurogenesis. These DCs produced neurotrophin-3 and activated endogenous microglia in the injured spinal cord. Behavioral analysis revealed the locomotor functions of DC-implanted mice to have recovered significantly as compared to those of control mice. Our results suggest that DC-implantation exerts trophic effects, including activation of endogenous NSPCs, leading to repair of the injured adult spinal cord. (C) 2004 Wiley-Liss, Inc.