FORMATION OF EXTRACELLULAR-MATRIX IN NORMAL RAT-LIVER - LIPOCYTES AS A MAJOR SOURCE OF PROTEOGLYCAN
FORMATION OF EXTRACELLULAR-MATRIX IN NORMAL RAT-LIVER - LIPOCYTES AS A MAJOR SOURCE OF PROTEOGLYCAN
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DOI：
10.1016/0016-5085(88)90502-1
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发表时间：
1988-08-01
期刊：
影响因子：
29.4
通讯作者：
BISSELL, DM
中科院分区：
文献类型：
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作者：
ARENSON, DM;FRIEDMAN, SL;BISSELL, DM
蛋白聚糖是正常肝细胞外基质的主要成分，在肝纤维化过程中发生质和量的变化。蛋白聚糖的细胞来源尚未完全确定。我们研究了这个问题，使用原代培养的肝细胞和脂肪细胞分离正常大鼠肝脏。通过测量硫酸化糖胺聚糖（蛋白聚糖的多糖部分）的产生来评估蛋白聚糖合成。研究结果表明，脂肪细胞产生的糖胺聚糖比肝细胞多六倍。三分之二的新合成物质与细胞或基质相关。在由脂肪细胞产生的单个糖胺聚糖种类中，硫酸皮肤素占总量的60%;硫酸乙酰肝素和硫酸软骨素是可测量的，但相对较少。在肝细胞培养物中，硫酸乙酰肝素基本上占检测到的所有糖胺聚糖。我们的结论是脂肪细胞是正常肝脏蛋白多糖的重要来源，可能是与肝纤维化相关的硫酸皮肤素的主要来源。
Proteoglycans are a major component of the normal hepatic extracellular matrix and undergo quantitative and qualitative changes in hepatic fibrosis. The cellular sources of proteoglycans are as yet incompletely defined. We examined this question using primary cultures of hepatocytes and lipocytes isolated from normal rat liver. Proteoglycan synthesis was assessed by measuring production of sulfated glycosaminoglycan, the polysaccharide moiety of proteoglycans. The findings indicate that lipocytes produce sixfold more glycosaminoglycan, per cell, than do hepatocytes. Two-thirds of the newly synthesized material is cell- or matrix-associated. Of the individual glycosaminoglycan species produced by lipocytes, dermatan sulfate represents 60% of the total; heparan sulfate and chondroitin sulfate was measurable but relatively minor. In hepatocyte cultures, heparan sulfate accounted for essentially all of the glycosaminoglycan detected. We conclude that lipocytes are an important source of proteoglycan in normal liver and may be the principal source of dermatan sulfate associated with hepatic fibrosis.