AN ELECTROPHORETIC KARYOTYPE FOR YEAST
AN ELECTROPHORETIC KARYOTYPE FOR YEAST
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DOI：
10.1073/pnas.82.11.3756
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
OLSON, MV
中科院分区：
文献类型：
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作者：
CARLE, GF;OLSON, MV
通过正交场交替凝胶电泳将标准实验室酿酒酵母菌株的染色体 DNA 分子分成 12 个分辨率良好的条带。 DNA .cntdot。来自克隆基因的 DNA 杂交探针被用来将这种条带模式与酵母基因定义的染色体相关联。显示 12 个条带代表 9 个单联体和 3 个共迁移双联体，从而说明了被鉴定为 I-XI 和 XIII-XVI 的 15 条染色体。因为在某些实验室酵母菌株中可以很容易地解析出 3 个共迁移双联体，这些酵母菌株相对于标准菌株含有染色体长度多态性，所以所有 15 条染色体可以在至少 4 个所研究的菌株中显示为单个条带。已知含有 rRNA 基因的第 16 条染色体（编号 XII）不会重复地进入凝胶。通过利用条带鉴定，先前未定位的片段 F8 被分配至 XIII 号染色体。除了尺寸或电泳行为与所有已知染色体有很大差异的染色体之外，结果似乎定义了酵母的完整“电泳核型”。
The chromosomal DNA molecules of a standard laboratory strain of Saccharomyces cerevisiae were separated into 12 well-resolved bands by orthogonal-field-alternation gel electrophoresis. DNA .cntdot. DNA hybridization probes derived from cloned genes were used to correlate this banding pattern with yeast''s genetically defined chromosomes. The 12 bands are shown to represent 9 singlets and 3 comigrating doublets, thereby accounting for 15 chromosomes that were identified as I-XI and XIII-XVI. Because the 3 comigrating doublets could be readily resolved in certain laboratory yeast strains that contain chromosome-length polymorphisms relative to the standard strain, all 15 of these chromosomes could be displayed as a single band in at least of 4 strains that were studied. A 16th chromosome (number XII), which is known to contain the genes for rRNA, does not reproducibly enter the gels. By making use of the band identifications, the previously unmapped fragment F8 was assigned to chromosome XIII. With the possible exception of chromosomes that differ greatly in size or electrophoretic behavior from all the known chromosomes, the results appear to define a complete "electrophoretic karyotype" for yeast.