Drought induces fructan synthesis and 1-SST (sucrose: sucrose fructosyltransferase) in roots and leaves of chicory seedlings (Cichorium intybus L.)
Drought induces fructan synthesis and 1-SST (sucrose: sucrose fructosyltransferase) in roots and leaves of chicory seedlings (Cichorium intybus L.)
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DOI：
10.1007/s004250050683
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发表时间：
2000-04-01
期刊：
影响因子：
4.3
通讯作者：
Van den Ende, W
中科院分区：
文献类型：
--
作者：
De Roover, J;Vandenbranden, K;Van den Ende, W
菊苣种子[医]叶状菌在酸洗过的蛭石中播种闪光，并在可控环境的生长室中生长。经过1个月的生长，植株不含蔗糖：蔗糖1-果糖基转移酶(1-SST)，这是果聚糖生物合成的关键酶。未观察到果聚糖。其中一些植物遭受了两周的干旱。胁迫植株根和叶中葡萄糖、果糖和蔗糖的浓度升高，根和叶片中的果糖浓度比对照高10倍。根中果聚糖合成的开始与1-SST活性的增加相一致。1-SST基因在胁迫植株的根和叶中均有表达。
Seeds of Cichorium intybus L. var. foliosum cv. Flash were sown in acid-washed vermiculite and grown in a controlled-environment growth chamber. After 1 month of growth, plantlets did not contain sucrose:sucrose 1-fructosyltransferase (1-SST), the key enzyme in fructan biosynthesis. No fructan could be observed. Some of the plants were submitted to drought for 2 weeks. Glucose, fructose and sucrose concentrations increased in roots and leaves of stressed plants and the fructan concentration in roots and leaves was ten times higher than in control plants. The onset of fructan synthesis coincided with the increase in 1-SST activity in roots. Expression of the 1-SST gene could be observed in roots and leaves of stressed plants.