Increased Akt and phosphorylated Akt expression are associated with malignant biological features of prostate cancer in Japanese men
Increased Akt and phosphorylated Akt expression are associated with malignant biological features of prostate cancer in Japanese men
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DOI：
10.1111/j.1464-410x.2007.07014.x
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发表时间：
2007-09-01
影响因子：
4.5
通讯作者：
Ogawa, Osamu
中科院分区：
文献类型：
--
作者：
Shimizu, Yousuke;Segawa, Takehiko;Ogawa, Osamu
探讨Akt蛋白表达与乳腺癌的关系（在肿瘤发生中起核心作用的丝氨酸/苏氨酸激酶）、磷酸化Akt（p-Akt）、前列腺癌肿瘤等级、雄激素受体（AR）染色评分，和Ki 67标记指数（LI）。通过石蜡切片的免疫组化染色分析细胞存活蛋白Akt的表达和活化。来自52名接受根治性前列腺切除术的日本男性的前列腺癌的包埋组织微阵列切片。Akt和p-Akt的表达与原发性Gleason分级、AR表达和Ki-67 LI的相关性分析表明，Akt和p-Akt的表达与原发性Gleason分级呈正相关（Fisher精确检验，P = 0.002和P = 0.032）。AR染色评分和Ki 67 LI均与Akt（P < 0.001）和p-Akt（P <0.001和P = 0.008）表达呈正相关。Akt和p-Akt的表达呈正相关（斯皮尔曼相关系数r = 0.644，P < 0.001），Akt和p-Akt的表达均与肿瘤分级、AR评分和Ki 67指数相关。这些数据表明Akt和p-Akt可能是检测日本人群前列腺癌恶性生物学特征的分子标志物。
To investigate the relationship between the expression of Akt (a serine/threonine kinase that plays a central role in tumorigenesis), phosphorylated Akt (p-Akt), prostate cancer tumour grade, androgen receptor (AR)-staining score, and Ki67 labelling index (LI) in Japanese men.The expression and activation of the cell survival protein Akt was analysed by immunohistochemical staining of paraffin-embedded tissue microarray sections of prostate carcinoma taken from 52 Japanese men who under radical prostatectomy. The correlation between the expression of Akt and p-Akt, and their relationship to primary Gleason grade, AR expression and Ki-67 LI was investigated.The expression of Akt and p-Akt were positively related to primary Gleason grade (Fisher's exact test, P = 0.002 and P = 0.032, respectively). Both AR-staining score and Ki67 LI were were positively related to the expression of Akt (both P < 0.001) and p-Akt (P < 0.001 and P = 0.008, respectively). There was a significant positive correlation between the expression of Akt and p-Akt (Spearman's correlation, r = 0.644, P < 0.001).Increased expression of both Akt and p-Akt were associated with higher tumour grade as well as a higher AR-staining score and Ki67 LI. These data indicate that Akt and p-Akt might be molecular markers for detecting malignant biological features of prostate cancer in the Japanese population.