A simple novel device for air sampling by electrokinetic capture
A simple novel device for air sampling by electrokinetic capture
复制标题
DOI：
10.1186/s40168-015-0141-2
复制
发表时间：
2015-12-27
期刊：
影响因子：
15.5
通讯作者：
Gilbert, Jack A.
中科院分区：
文献类型：
--
作者：
Gordon, Julian;Gandhi, Prasanthi;Gilbert, Jack A.
背景：目前有多种不同的采样装置可获取空气样本以研究空气微生物组。所有这些都具有一定程度的技术复杂性，限制了部署。在这里，我们评估了一种新型设备的使用，该设备没有技术复杂性并且易于部署。结果：由动电推进驱动的空气清洁设备已被采用，以提供一种收集有氧生物组样本的通用方法。气溶胶颗粒中的等离子体感应电荷导致对电极的推进和捕获。离子流产生净大量气流，没有移动部件。设备和电极组件根据空气净化技术进行了重新设计，可提供 120 lpm 的平均空气流量。这与当前基于物理抽气的空气采样装置相比具有优势。捕获效率是通过在受控环境室中使用荧光乳胶颗粒与 0.4 μm 聚碳酸酯参考过滤器进行比较来确定的。在三种不同环境下的现场研究中，将性能与相同的参考过滤方法进行了比较。对于 23 种常见真菌，通过定量聚合酶链式反应 (qPCR) 检测，灵敏度为 100%，表观特异性为 87%，参考滤光片被视为“金标准”。此外，通过扩增子测序对 16S RNA 进行细菌分析，结果显示动电装置和参考过滤器捕获的群落结构相同。与当前其他空气采样方法不同，颗粒的捕获是由电荷决定的，因此不受颗粒质量控制。我们分析了从空气中捕获的颗粒尺寸，不考虑原子力显微镜的特定分析物：可以从环境空气中捕获至少低至 100 nM 的颗粒。结论：这项工作介绍了一种非常简单的即插即用设备，可以在没有移动部件的情况下以高容量流速对空气进行采样，并收集小至亚微米范围的颗粒。该设备的性能基本上相当于通过过滤器进行捕获，通过定量 PCR 和扩增子测序进行微生物组分析。
Background: A variety of different sampling devices are currently available to acquire air samples for the study of the microbiome of the air. All have a degree of technical complexity that limits deployment. Here, we evaluate the use of a novel device, which has no technical complexity and is easily deployable.Results: An air-cleaning device powered by electrokinetic propulsion has been adapted to provide a universal method for collecting samples of the aerobiome. Plasma-induced charge in aerosol particles causes propulsion to and capture on a counter-electrode. The flow of ions creates net bulk airflow, with no moving parts. A device and electrode assembly have been re-designed from air-cleaning technology to provide an average air flow of 120 lpm. This compares favorably with current air sampling devices based on physical air pumping. Capture efficiency was determined by comparison with a 0.4 mu m polycarbonate reference filter, using fluorescent latex particles in a controlled environment chamber. Performance was compared with the same reference filter method in field studies in three different environments. For 23 common fungal species by quantitative polymerase chain reaction (qPCR), there was 100 % sensitivity and apparent specificity of 87 %, with the reference filter taken as "gold standard." Further, bacterial analysis of 16S RNA by amplicon sequencing showed equivalent community structure captured by the electrokinetic device and the reference filter. Unlike other current air sampling methods, capture of particles is determined by charge and so is not controlled by particle mass. We analyzed particle sizes captured from air, without regard to specific analyte by atomic force microscopy: particles at least as low as 100 nM could be captured from ambient air.Conclusions: This work introduces a very simple plug-and-play device that can sample air at a high-volume flow rate with no moving parts and collect particles down to the sub-micron range. The performance of the device is substantially equivalent to capture by pumping through a filter for microbiome analysis by quantitative PCR and amplicon sequencing.