Effect of dietary clofibrate on epoxide hydrolase activity in tissues of mice.
Effect of dietary clofibrate on epoxide hydrolase activity in tissues of mice.
复制标题
膳食安妥明对小鼠组织中环氧化物水解酶活性的影响。
DOI：
10.1016/0006-2952(85)90656-2
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发表时间：
1985
影响因子：
5.8
通讯作者：
Hammock,BD
中科院分区：
文献类型：
--
作者：
Loury,DN;Moody,DE;Kim,BW;Hammock,BD
以反式二苯乙烯氧化物为胞浆环氧化物水解酶的选择性底物，顺式二苯乙烯氧化物和苯并[a]芘4，5-氧化物为微粒体环氧化物水解酶的底物，顺式二苯乙烯氧化物为谷胱甘肽S-转移酶的底物，研究了氯贝特对小鼠肝、肾、肺和睾丸环氧化物代谢酶活性的影响。反式二苯乙烯氧化物的水合作用在肝脏中最大，其次是肾脏>肺>睾丸。其水解显着增加，在细胞溶质部分的肝和肾的氯贝酯治疗的小鼠，并在微粒体从肝脏。等电聚焦表明，相同的酶是负责水解的反式二苯乙烯氧化物在正常和诱导的肝脏和肾脏。氯贝特诱导谷胱甘肽S-转移酶活性的顺式二苯乙烯氧化物仅在肝脏。顺式二苯乙烯氧化物和苯并[a]芘4，5-氧化物在睾丸中的水解率最高，其次是肝脏>肺>肾脏。氯贝丁酯可显著诱导肝和肾中顺式二苯乙烯氧化物的水合作用，而苯并[a]芘4，5-氧化物仅在肝中诱导。基于正常和诱导动物组织中苯并[a]芘4，5-氧化物与顺式-二苯乙烯氧化物的水合比率的这些和其他数据表明，存在一种或多种新型环氧化物水解酶活性，其不能由经典的胞质或微粒体水解酶来解释。这些作用在肾微粒体中特别是在睾丸的胞质中是显著的。
The effects of dietary clofibrate on the epoxide-metabolizing enzymes of mouse liver, kidney, lung and testis were evaluated usingtrans-stilbene oxide as a selective substrate for the cytosolic epoxide hydrolase,cis-stilbene oxide and benzo[a]pyrene 4,5-oxide as substrates for the microsomal form, andcis-stilbene oxide as a substrate for glutathioneS-transferase activity. The hydration oftrans-stilbene oxide was greatest in liver followed by kidney > lung > testis. Its hydrolysis was increased significantly in the cytosolic fraction of liver and kidney of clofibrate-treated mice and in the microsomes from the liver. Isoelectric focusing indicates that the same enzyme is responsible for hydrolysis oftrans-stilbene oxide in normal and induced liver and kidney. Clofibrate induced glutathioneS-transferase activity oncis-stilbene oxide only in the liver. Hydrolysis of bothcis-stilbene oxide and benzo[a]pyrene 4,5-oxide was highest in testis followed by liver>lung>kidney. Hydration ofcis-stilbene oxide was induced significantly in both liver and kidney by clofibrate but that of benzo[a]pyrene 4,5-oxide was induced only in the liver. These and other data based on ratios of hydration of benzo[a]pyrene 4,5-oxide tocis-stilbene oxide in tissues of normal and induced animals indicate that there are one or more novel epoxide hydrolase activities which cannot be accounted for by either the classical cytosolic or mirosomal hydrolases. These effects are notable in the microsomes of kidney and especially in the cytosol of testis.