Ischemia-reperfusion injury of adipofascial tissue: an experimental study evaluating early histologic and biochemical alterations in rats.
Ischemia-reperfusion injury of adipofascial tissue: an experimental study evaluating early histologic and biochemical alterations in rats.
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DOI：
10.1155/mi.2005.304
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发表时间：
2005-10-24
影响因子：
4.6
通讯作者：
Ciralik H
中科院分区：
文献类型：
--
作者：
Coban YK;Kurutas EB;Ciralik H
脂肪坏死仍然是皮瓣重建的严重并发症。在临床环境中，众所周知，脂肪组织更容易发生缺血事件。我们的目的是评估在实验模型中的脂肪筋膜组织的早期组织学和生化变化。采用大鼠上腹部皮瓣模型，观察缺血再灌注（I-R）对脂肪筋膜组织的影响。两组动物（一组仅缺血，另一组缺血再灌注组）用于评估组织学水肿，充血和血管外出血的程度，以及脂肪筋膜瓣内的早期生化改变。生化指标包括谷胱甘肽（GSH）和丙二醛（MDA）。各组均取对侧腹股沟皮下脂肪组织作为对照。将这些评价与正常未处理的对侧腹部皮下脂肪组织进行比较。缺血再灌注组皮瓣水肿、充血、出血明显多于对照组（P <0.0001）。对照组脂肪组织水肿程度较单纯缺血组轻（P <0.05）。单纯缺血组皮瓣出血量及水肿程度均明显低于缺血再灌注组（P <0.001）。MDA/GSH比值对照组为33，单纯缺血组为37，缺血再灌注组为82。本研究证实，脂肪组织在缺血和缺血-再灌注事件后发生显著的组织学和生物化学改变。I-R后脂肪组织抗氧化剂水平显著下降，这表明在临床环境中应采取预防措施。
Fat necrosis remains a serious complication in reconstructive flaps. In clinical setting, it is well known that fat tissue is more susceptible to ischemic events. We aimed to evaluate early histological and biochemical changes of adipofascial tissue in an experimantal model. An epigastric flap model in rats was used to evaluate the effect of ischemia-reperfusion (I-R) injury on adipofascial tissue. Two groups of animals (one with ischemia alone and other ischemia-reperfusion group) were used to evaluate the degree of histological edema, congestion and extravascular bleeding, and early biochemical alterations within the adipofascial flaps. The biochemical parameters included glutathione (GSH) and malondialdehyde (MDA). In each group, contralateral groin subcutaneous adipose tissue served as control. These evaluations were compared to normal unmanipulated, contralateral abdominal subcutaneous adipose tissue. The ischemia-reperfused flap group showed histologically significantly much edema congestion and bleeding than the control groups (P < .0001). The control group showed less edema in fat tissue than the ischemia-alone group (P < .05). All of the flaps in the ischemia-only group showed significantly less bleeding and edema than I-R group (P < .001). The ratio of MDA/GSH was 33 in control, 37 in ischemia alone, and 82 in ischemia-reperfusion groups, respectively. This study confirms that significant histologic and biochemical alteration occurs after ischemia and ischemia-reperfusion events in adipose tissue. Marked drop in adipose tissue antioxidant levels after I-R suggested that preemptive measures to this decrease should be undertaken in clinical settings.